应用特征光合色素研究东海和南海北部浮游植物的群落结构

doi:10.11867/j.issn.1001-8166.2006.07.0738

应用高效液相色谱并结合二极管阵列检测器分析技术，研究了东海与南海北部典型断面浮游植物的光合色素组成，进而由CHEMTAX软件估算浮游植物群落结构。结果表明：东海断面浮游植物叶绿素a生物量大于南海北部断面。受海水层化结构影响，东海PN断面浮游植物群落结构形成上层和下层两种类型，上层以蓝藻为主要优势类群；下层以硅藻为主要优势类群。南海北部S断面浮游植物群落形成近岸与离岸两种类型，近岸以硅藻、隐藻、绿藻为主要优势类群；离岸以定鞭金藻、蓝藻、原绿球藻为主要优势类群。初步分析了研究海区浮游植物群落结构与环境因子的关系。

关键词: 群落结构, 东海, 南海北部, 光合色素, 浮游植物

Phytoplankton samples from typical stations along the East China Sea and the northern south China sea were analyzed by RP-HPLC combined with DAD (diode array detector) to determine pigments composition. Ocean study included PN section along East China sea(September in 2002) and northern of south China sea(November in 2002). Pigments data were transformed into phytoplankton composition by software CHEMTAX based on least square method and steepest descent algorithm. This work showed that phytoplankton communities in PN transect were clustered into two types, mostly influenced by stratification. Communites in upper layer were dominated by cyanobacteria, while diatoms were most important groups in lower layer. Also two types of phytoplankton community structure in S transect were clustered by the distance from the shore. Diatoms, Cryptophyta, Chlorophyta were main groups in shore, and Prymnesiophyta, Cyanobacteria, Prochlorophyta were main groups offshore. Additionally,primary analysis was carried out to illuminate the relationship of phytoplankton community structure and environmental factors.

Key words: Photosynthetic pigment, Phytoplankton, Community structure, East China sea, Northern south China sea.

中图分类号:

Q178.53

[1] Simon N, Barlow R G, Marie D, et al. Characterization of oceanic photosynthetic picoeukaryotes by flow cytometry[J]. Journal of Phycology,1994,30:922-935.

[2] Barlow R G, Mantoura R F C, Gough M A, et al. Pigment signatures of the phytoplankton composition in the northeastern Atlantic during the 1990 spring bloom[J]. Deep-Sea Research II,1993,40:459-477.

[3] Wright S W, Thomas D P, Marchant H J, et al. Analysis of phytoplankton of the Australian sector of the Southern Ocean: Comparisons of microscopy and size frequency data with interpretations of pigment HPLC data using the CHEMTAX' matrix factorisation program[J]. Marine Ecology Progress Series,1996,144:285-298.

[4] Andersen R A, Bidigare R R, Keller M D, et al. A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific Oceans[J]. Deep-Sea Research II,1996,43:517-537.

[5] Gieskes W W C, Kraay G W. Dominance of Cryptophyceae during the phytoplankton spring bloom in the central North Sea detected by HPLC analysis of pigments[J]. Marine Biology,1983,75:179-185.

[6] Letelier R M, Bidigare R R, Hebel D V, et al. Temporal variability of phytoplankton community structure based on pigment analysis[J]. Limnology and Oceanography,1993,38:1 420-1 437.

[7] Laurence D-S, Georges B, Bertrand Le. A chemotaxonomic method to quantify phytoplankton groups in freshwater lentic mesocosms: An approach including chlorophyll a breakdown products[J]. C. R. Biologies,2003,326:95-105.

[8] Mackey M D, Mackey D J, Higgins H W, et al. CHEMTAX-A program for estimating class abundances from chemical markers: Application to HPLC measurements of phytoplankton[J]. Marine Ecology Progress Series, 1996, 144:265-283.

[9] Furuya K, Masato H, Yasushi Y, et al. Phytoplankton dynamics in the East China Sea in spring and summer as revealed by HPLC-derived pigment signatures[J]. Deep-Sea Research II, 2003, 50(3):367-387.

[10] Walsh J J, Wirick C D, Pietrafesa L J, et al. High-frequency sampling of the 1984 spring bloom within the Mid-Atlantic Bight: Synoptic shipboard, aircraft and in situ perspectives of the SEEP-I experiment[J]. Continental Shelf Research,1988,8:529-563.

[11] Jing Zhang. Biogeochemistry of chinese estuarine and coastal waters: Nutrients, trace metals and biomarkers[J]. Region Environmental Change,2002, 3:65-76.

[12] Gu Xingen, Yuan Qi, Yang Jiaowen, et al. Study on vertical distribution of phytoplankton in the water of changjiang estuang[J]. Journal of Fishery Sciences of China,1995,2(1):28-38.[顾新根,袁骐,杨蕉文,等.长江口外水域浮游植物垂直分布研究[J].中国水产科学,1995,2(1): 28-38.]

[13] Hsiu-Ping Li, Gwo-Ching Gong, Tung-Ming Hsiung. Phytoplankton pigment analysis by HPLC and its application inalgal community investigations[J]. Botanical Bulletia of Academy Sinica,2002,43:283-290.

[14] Furuya K, Hayashi M, Yabushita Y. HPLC Determination of Phytoplankton Pigments Using N,N-Dimethyl-formamide [J]. Journal of Oceanography,1998,54:199-203.

[15] Laurie V H, Crypstal S T. Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments[J]. Journal of Chromatography, 2001,910:31-49.

[16] Joanna S. Qualitative and quantitative analysis of Baltic phytoplankton pigments[J]. Oceanologia,2000,42 (4):449-471.

[17] Chen Jixin,Huang Bangqin,Jia Xiwei,et al. Community structure of marine ultraphytoplankton in xiamen water using photosynthetic pigments[J]. Marine Environmental Science,2003,22(3):16-21.[陈纪新,黄邦钦,贾锡伟,等.利用光合色素研究厦门海域超微型浮游植物群落结构[J].海洋环境科学,2003, 22(3):16-21.]

[18] Vinay P S, Constatin A R. Chloroplast biogenesis: Determination of the molar extinction coefficient of divinyl chlorophyll a and b and their pheophytin[J]. Analytic Biochemistry,1992,207:261-266.

[19] Gao Yahui, Yu Qiubo, Qi Yuzao, et al. Speries composition and ecological distribution of planktnic diatoms in the Changjiang river estuary during spring[J]. Chinese Journal of Applied Ecology,2003,14(7):1 044-1 048.[高亚辉,虞秋波,齐雨藻,等.长江口附近海域春季浮游硅藻的种类组成和生态分布[J].应用生态学报,2003,14(7)∶1 044-1 048.]

[20] Wu Yulin, Fu Yuena, Zhang Yongshan, et al. Phutoplankton distributionand its relation to the runoft in the Changjiang estuary[J]. Oceanologic Etlimaologia Sinia,2004,35(3):246-251.[吴玉霖,傅月娜,张永山,等.长江口海域浮游植物分布及其与径流的关系[J].海洋与湖沼,2004,35(3):246-251.]

[21] Wu Y L, Zhang Y S, Zhou C X. Phytoplankton distribution and community structure in the east China sea (ECS) continental shelf[J]. Chinese Journal of Oceanology and Limnology,2000,18(1):74-79.

[22] 林金美. 东海浮游甲藻类的分布[J].海洋学报,1994,(2):110-115.

[23] Lu Douding, Jeanette G. Study on taconomy and distribution of Ornithocercas in the East China Sea[J]. Donghai Marine Science,2001,19(3):11-18.[陆斗定, Jeanette G. 东海陆架黑潮区鸟尾藻的分类及其生态分布特点[J].东海海洋,2001,19(3):11-18.]

[24] Yang Qingliang. Characteristics of Trichodesmium distribution in waters over continental shelves of the South Huanghai Sea and the East China Sea[J]. Acta Oceanologica Sinica,1998,20(5):93-100.[杨清良.南黄海和东海陆架区束毛藻(Trichodesmium)的分布特征[J].海洋学报1998,20(5):93-100.]

[25] Veldhuis M J, Kraay G W. Phytoplankton in the subtrophic Atlantic Ocean: Towards a better assessment of biomass and composition[J]. Deep-Sea Research I, 2004, 51:507-530.

[26] Furuya K. Subsurface chlorophyll maximum in the tropical and subtropical western Pacific Ocean: Vertical profiles of phytoplankton biomass and its relationship with chlorophyll a and particulate organic carbon[J]. Marine Biology,1990,107:529-539.

[27] Chen Y L L. Summer phytoplankton community structure in the Kuroshio current-related upwelling northeast of Taiwan[J]. TAO,1992,3(3): 305-320 .

[28] Takahashi K, Fujitani N, Yanada M, et al. Five year long particle fluxes in the central subarctic Pacific and the Bering Sea[C]∥Tsunogai S,ed. Biogeochemical Processes in the North Pacific. Tokyo: Japan Marine Science Foundation, 1997:277-289.

[29] Yu Fei, Zang Jiaye, Guo Binghuo, et al. Some phenomena of the kuroshio intrasion in to Shelt area and shelf circulation of the East China Sea[J]. Advances in Marine Science,2002,20(3):22-28.[于非,臧家业,郭炳火,等.黑潮水入侵东海陆架及陆架环流的若干现象[J].海洋科学进展,2002,20(3): 22-28.]

[30] Huang L M, Jian W J, Song X Y, et al. Species diversity and distribution for phytoplankton of the Pearl River estuary during rainy and dry seasons[J]. Marine Pollution Bulletin,2004,49: 588-596.

[31] Zhu Genhai,Ning Xiuren, Cai Yuming, et al. Studies on species composition and abundance distribution of phytoplankton in the South China Sea[J]. Acta Oceanologica Sinica,2003,25:9-23.[朱根海,宁修仁,蔡昱明,等.南海浮游植物种类组成和丰度分布的研究[J].海洋学报,2003,25:9-23.]

[32] Cortes M Y, Bollmann J, Thierstein H R. Coccolithophore ecology at the HOT station ALOHA, Hawaii[J]. Deep-Sea Research II,2001,48:1 957-1 981.

[33] Millie D F,Paerl H W,Hurley J P. Microalgal pigment assessments using high-performance liquid chromatography: A synopsis of organismal and ecological applications[J]. Canadian Journal of Fisheries and Aquatic Sciences,1993,50:2 513-2 527.

[34] Millie D F,Kirkpatrick G J,Vinyard B T. Relating photosynthetic pigments and in vivo optical density spectra to irradiance for the Florida red-tide dinoflagellate Gymnodinium breve[J]. Marine Ecology Progress Series, 1995, 120:65-75.

[35] Jeffrey S W, Wright S W. Qualitative and quantitative HPLC analysis of SCOR reference algal cultures[C]∥Jeffrey S W, Mantoura R F C, Wright S W, eds. Phytoplankton Pigments in Oceanography. Paris:UNESCO Press, 1997:343-360.

[36] Goericke R, Montoya J P. Estimating the contribution of microalgal taxa to chlorophyll a in the field-variations of pigment ratios under nutrient- and light-limited growth[J]. Marine Ecology Progress Series,1998,169:97-112.

[37] Schluter L,Mohlenberg F,Havskum H,et al. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas:Testing the influence of light and nutrients on pigment/chlorophyll a ratios[J]. Marine Ecology Progress Series,2000,192:49-63.

[38] Mackey D J, Higgins H W, Mackey M D, et al. Algal class abundance in the western equatorial Pacific: Estimation from HPLC pigments using CHEMTAX[J]. Deep-Sea Research I,1998,45:1 441-1 468.

[39] Wright S W, Enden R L V D. Phytoplankton community structure and stocks in the East Antarctic marginal ice zone (BROKE survey, January March 1996) determined by CHEMTAX analysis of HPLC pigment signatures[J]. Deep-Sea Research II,2000,47:2 363-2 400.

[1]	殷怡童,罗锡明. 含铁介质稳定砷与根际微生物的相互作用[J]. 地球科学进展, 2020, 35(10): 1052-1063.
[2]	张成晨,许长海,何敏,高顺莉. 东海到南海晚中生代岩浆弧及陆缘汇聚体制综述[J]. 地球科学进展, 2019, 34(9): 950-961.
[3]	王萌,张艳伟,刘志飞,吴家望. 南海北部中尺度涡的时空分布特征：基于卫星高度计资料的统计分析[J]. 地球科学进展, 2019, 34(10): 1069-1080.
[4]	王芳慧, 陈莹, 王波, 李好文, 周升钱. 海洋微生物气溶胶的丰度、群落结构及影响机制[J]. 地球科学进展, 2018, 33(8): 783-793.
[5]	祁建华, 李孟哲, 高冬梅, 甄毓, 张大海. 沙尘天气对大气生物气溶胶中微生物浓度、特性和分布的影响[J]. 地球科学进展, 2018, 33(6): 568-577.
[6]	邓浩俊, 陶贞, 高全洲, 姚玲, 冯雍, 李银花. 河流筑坝对生源物质循环的改变研究进展 ^*[J]. 地球科学进展, 2018, 33(12): 1237-1247.
[7]	李佳霖, 秦松. 海洋微微型蓝细菌分子生态学研究进展[J]. 地球科学进展, 2015, 30(4): 477-486.
[8]	黄邦钦, 柳欣. 边缘海浮游生态系统对生物泵的调控作用[J]. 地球科学进展, 2015, 30(3): 385-395.
[9]	卢汐, 宋金明, 袁华茂, 李宁. 黑潮与毗邻陆架海域的碳交换[J]. 地球科学进展, 2015, 30(2): 214-225.
[10]	曲宝晓, 宋金明, 袁华茂, 李学刚, 李宁, 段丽琴,马清霞, 陈鑫. 东海海—气界面二氧化碳通量的季节变化与控制因素研究进展[J]. 地球科学进展, 2013, 28(7): 783-793.
[11]	金杰，刘素美. 海洋浮游植物对磷的响应研究进展[J]. 地球科学进展, 2013, 28(2): 253-261.
[12]	郑勇玲，吴承强，蔡锋，鲍晶晶，许艳. 我国海底地貌研究进展及其在东海近海的新发现、新认识[J]. 地球科学进展, 2012, 27(9): 1026-1034.
[13]	王国栋, 康建成, Han Guoqi, 刘超, 闫国东. 中国东海海平面变化多尺度周期分析与预测[J]. 地球科学进展, 2011, 26(6): 678-684.
[14]	宋洪军，季如宝，王宗灵. 近海浮游植物水华动力学和生物气候学研究综述[J]. 地球科学进展, 2011, 26(3): 257-265.
[15]	丁玲，邢磊，赵美训. 生物标志物重建浮游植物生产力及群落结构研究进展[J]. 地球科学进展, 2010, 25(9): 981-989.

阅读次数

全文

摘要

Phytoplankton Community Structure in the Transects across East China Sea and Northern South China Sea Determined by Analysis of HPLC Photosynthetic Pigment Signatures