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地球科学进展  2017, Vol. 32 Issue (11): 1193-1203    DOI: 10.11867/j.issn.1001-8166.2017.11.1193
综述与评述     
生物标志物单体放射性碳同位素分析技术的发展
张海龙1(), 陶舒琴2,*(), 于蒙1, 赵美训1
1.中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100
2.国家海洋局海洋大气化学与全球变化重点实验室,福建 厦门 361005
A Review on Techniques and Applications of Biomarker Compound-specific Radiocarbon Analysis
Hailong Zhang1(), Shuqin Tao2,*(), Meng Yu1, Meixun Zhao1
1.Key Laboratory of Marine Theory and Technology, Ministry of Education, Ocean University of China,Qingdao 266100, China
2.Key Laboratory of Global Change And Marine-Atmospheric Chemistry of State Oceanic Administration, Third Institute of Oceanography, Xiamen 361005, China
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摘要:

自Eglinton等(1996)首次将单体放射性碳同位素分析技术(CSRA)应用于海洋沉积物中生物标志物14C研究以来,该技术发展迅速,广泛应用于海洋科学、生物地球化学和古气候学等领域。但是,自然环境样品中生物标志物大多含量低、干扰基质复杂而且难以分离。因此,如何从基质组成复杂的样品中分离富集高纯度的目标化合物已经成为限制CSRA技术发展和应用的瓶颈。近些年来,不断改进的色谱分离富集技术不仅提高了目标化合物分离的纯度和回收效率,同时也扩展了生物标志物的碳同位素示踪应用。综合介绍了自然环境研究中单体化合物放射性碳同位素分析技术中常见生物标志物单体分离纯化的技术方法以及发展现状。

关键词: 单体放射性碳同位素生物标志物制备气相色谱制备液相色谱    
Abstract:

Eglinton (1996) firstly performed the Compound-Specific Radiocarbon Analysis (CSRA) in marine sediments, providing a new approach to understanding the organic carbon source. The applications of CSRA technique were developed rapidly and widely used in oceanography, biogeochemistry and paleoclimatology. However, because of the extremely low amount and interference with complex substrates, mostly source-specific biomarkers were hard to be separated from environmental matrix samples. Therefore, how to harvest pure biomarkers, from complex natural sample matrixes, has been the limitation of CSRA technique, especially for ultra-small size CSRA. Recently, various preparative chromatography techniques have been carried out to improve the purity and recovery efficiency of the target compounds. This paper aimed to introduce the techniques and applications of CSRA for common source-specific biomarkers in the natural environment.

Key words: Compound-Specific Radiocarbon Analysis (CSRA)    Biomarkers    Preparative Capillary Gas Chromatography(PCGC)    Prep-HPLC.
收稿日期: 2017-07-10 出版日期: 2018-01-10
ZTFLH:  P59  
基金资助: 国家海洋局第三海洋研究所科研业务费专项资金资助项目“有机(生物)标志物技术对海洋气溶胶来源示踪的研究”(编号:海三科2017013);国家自然科学基金项目“利用分子生物标志物——14C技术重建全新世黄海沉积有机质组成变化”(编号:41506087)资助
通讯作者: 陶舒琴     E-mail: zhanghailong@ouc.edu.cn;taoshuqin@tio.org.cn
作者简介:

作者简介:张海龙(1981-),男,山东即墨人,博士研究生,主要从事海洋有机地球化学研究.E-mail:zhanghailong@ouc.edu.cn

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张海龙, 陶舒琴, 于蒙, 赵美训. 生物标志物单体放射性碳同位素分析技术的发展[J]. 地球科学进展, 2017, 32(11): 1193-1203.

Hailong Zhang, Shuqin Tao, Meng Yu, Meixun Zhao. A Review on Techniques and Applications of Biomarker Compound-specific Radiocarbon Analysis. Advances in Earth Science, 2017, 32(11): 1193-1203.

链接本文:

http://www.adearth.ac.cn/CN/10.11867/j.issn.1001-8166.2017.11.1193        http://www.adearth.ac.cn/CN/Y2017/V32/I11/1193

图1  PCGC系统工作原理图示[26]
直接法 间接法 直接法 间接法 参考文献
Cchemistry Cpcgc Cchemistry Cpcgc Cchemistry+pcgc Cchemistry+pcgc
1.1 1.0 0.1±0.05 0.6±0.3 1.2 1.6 [38]
1.3±0.7 0.1±0.1 1.4±0.7 1.6±0.9 [57]
0.3±0.2 2.0±1.0 [57]
2.44±0.22 1.25±0.24 [58]
表1  不同实验室、不同时间湿化学分离(Cchemistry)及PCGC分离(Cpcgc)过程引入的外源碳(Cex)的比较(单位:μg C/min)
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