地球科学进展

地球科学进展 ›› 2017, Vol. 32 ›› Issue (11): 1193 -1203. doi: 10.11867/j.issn.1001-8166.2017.11.1193

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生物标志物单体放射性碳同位素分析技术的发展
张海龙 1( ), 陶舒琴 2, *( ), 于蒙 1, 赵美训 1   
  1. 1.中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东 青岛 266100
    2.国家海洋局海洋大气化学与全球变化重点实验室,福建 厦门 361005
  • 收稿日期:2017-07-10 修回日期:2017-10-02 出版日期:2017-11-10
  • 通讯作者: 陶舒琴 E-mail:zhanghailong@ouc.edu.cn;taoshuqin@tio.org.cn
  • 基金资助:
    国家海洋局第三海洋研究所科研业务费专项资金资助项目“有机(生物)标志物技术对海洋气溶胶来源示踪的研究”(编号:海三科2017013);国家自然科学基金项目“利用分子生物标志物—— 14C技术重建全新世黄海沉积有机质组成变化”(编号:41506087)资助

A Review on Techniques and Applications of Biomarker Compound-specific Radiocarbon Analysis

Hailong Zhang 1( ), Shuqin Tao 2, *( ), Meng Yu 1, Meixun Zhao 1   

  1. 1.Key Laboratory of Marine Theory and Technology, Ministry of Education, Ocean University of China,Qingdao 266100, China
    2.Key Laboratory of Global Change And Marine-Atmospheric Chemistry of State Oceanic Administration, Third Institute of Oceanography, Xiamen 361005, China
  • Received:2017-07-10 Revised:2017-10-02 Online:2017-11-10 Published:2018-01-10
  • Contact: Shuqin Tao E-mail:zhanghailong@ouc.edu.cn;taoshuqin@tio.org.cn
  • About author:

    First author:Zhang Hailong (1981-), male, Jimo City, Shandong Province, Ph.D student. Research areas include marine organic geochemistry.E-mail:zhanghailong@ouc.edu.cn

  • Supported by:
    Project supported by the Scientific Research Foundation of Third Institute of Oceanography, SOA “Source apportionment of molecular specific biomarkers from marine aerosols” (No.Haisanke2017013);The National Natural Science Foundation of China “The application of compound-specific biomarker 14C technique for reconstruction of the changes of sedimentary organic composition in the Yellow Sea during the Holocene” (No.41506087)
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自Eglinton等(1996)首次将单体放射性碳同位素分析技术(CSRA)应用于海洋沉积物中生物标志物14C研究以来,该技术发展迅速,广泛应用于海洋科学、生物地球化学和古气候学等领域。但是,自然环境样品中生物标志物大多含量低、干扰基质复杂而且难以分离。因此,如何从基质组成复杂的样品中分离富集高纯度的目标化合物已经成为限制CSRA技术发展和应用的瓶颈。近些年来,不断改进的色谱分离富集技术不仅提高了目标化合物分离的纯度和回收效率,同时也扩展了生物标志物的碳同位素示踪应用。综合介绍了自然环境研究中单体化合物放射性碳同位素分析技术中常见生物标志物单体分离纯化的技术方法以及发展现状。

关键词: 单体放射性碳同位素, 生物标志物, 制备气相色谱, 制备液相色谱

Eglinton (1996) firstly performed the Compound-Specific Radiocarbon Analysis (CSRA) in marine sediments, providing a new approach to understanding the organic carbon source. The applications of CSRA technique were developed rapidly and widely used in oceanography, biogeochemistry and paleoclimatology. However, because of the extremely low amount and interference with complex substrates, mostly source-specific biomarkers were hard to be separated from environmental matrix samples. Therefore, how to harvest pure biomarkers, from complex natural sample matrixes, has been the limitation of CSRA technique, especially for ultra-small size CSRA. Recently, various preparative chromatography techniques have been carried out to improve the purity and recovery efficiency of the target compounds. This paper aimed to introduce the techniques and applications of CSRA for common source-specific biomarkers in the natural environment.

Key words: Compound-Specific Radiocarbon Analysis (CSRA), Biomarkers, Preparative Capillary Gas Chromatography(PCGC), Prep-HPLC.

中图分类号: 

图1 PCGC系统工作原理图示 [ 26 ]
Fig.1 Diagrammatic representation of the PCGC instrument [ 26 ]
表1 不同实验室、不同时间湿化学分离(C chemistry)及PCGC分离(C pcgc)过程引入的外源碳(C ex)的比较(单位:μg C/min)
Table 1 The comparison of C ex is introduced between laboratories and period from the chemical separation (C chemistry) and PCGC separation (C pcgc) process (unit: μg C/min)
直接法 间接法 直接法 间接法 参考文献
Cchemistry Cpcgc Cchemistry Cpcgc Cchemistry+pcgc Cchemistry+pcgc
1.1 1.0 0.1±0.05 0.6±0.3 1.2 1.6 [38]
1.3±0.7 0.1±0.1 1.4±0.7 1.6±0.9 [57]
0.3±0.2 2.0±1.0 [57]
2.44±0.22 1.25±0.24 [58]
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