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地球科学进展  2015, Vol. 30 Issue (10): 1162-1171    DOI: 10.11867/j.issn.1001-8166.2015.10.1162
IODP研究     
完整极性脂质化合物对海洋微生物活动的指示及应用局限性
宋敏1, 2, 杨群慧2, *, 王华1, 季福武2, 王虎2, 潘安阳2, 周怀阳2
1.同济大学环境科学与工程学院,上海 200092; 2.同济大学海洋地质国家重点实验室,上海 200092
Applications of Intact Polar Lipids for Tracing the Marine Microbial Activity and Their Limitations
Song Min1, 2, Yang Qunhui2, *, Wang Hua1, Ji Fuwu2, Wang Hu2, Pan Anyang2, Zhou Huaiyang2
1. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; 2. State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, China
 全文: PDF(1097 KB)  
摘要:

回顾了近10年来完整极性脂质化合物(IPLs)在海洋微生物研究中的应用及存在的问题,并展望了IPLs应用的发展前景。作为新的热点之一,对海洋水体悬浮颗粒物中IPLs的研究,不仅推进了人们对水柱中IPLs分布和转化的了解,同时也深化了对古环境指标TEX86适用性的认识,有助于更好地开展古环境重建研究。色谱和质谱技术的发展,新型化合物的发现以及IPLs单体碳同位素分析的应用,使得IPLs在示踪海洋环境中真核生物与微生物共生作用、微生物介导的好氧和厌氧氨氧化以及甲烷厌氧氧化作用、微生物的代谢状态等方面也取得了重要研究进展。需要注意的是,由于不同的IPLs降解速率并不一致,其中部分古菌糖脂的降解周期可能远大于微生物群落的更新周期,因此可能并不适于表征活体生物量;同时,环境中氧气含量及有机质浓度也会对IPLs的降解速率造成影响;另外,在微生物不同的生长阶段,或者微生物由于生长环境条件变化产生生理响应时,IPLs组成也可能会发生变化。因此,应用IPLs指示微生物活动和反演古环境时应注意指标的适用性。

关键词: 完整极性脂质微生物活动海洋沉积物悬浮颗粒物局限性    
Abstract:

Intact Polar Lipids (IPLs) are synthesized predominately or uniquely by specific organisms and would degrade rapidly after cell death. Such biomarker IPLs can be used to indicate the microbial distribution and activity in marine environment. Here the progress of the aforementioned studies made over the last decade was reviewed. With the development of chromatography and mass spectrometry, the discovery of new IPLs compounds and the application of stable carbon isotope composition (δ13C) of IPLs, our understanding of the composition and transformation of IPLs in suspended particulate matter in the water column and of the applicability of the TEX86 proxy are greatly improved. Besides, IPLs are widely applied in the study of marine eukaryotes-bacteria symbiosis, aerobic and anaerobic ammonia oxidation, anaerobic methane oxidation and microbial metabolic states. Meanwhile, it is suggested by recent studies that different IPLs often exhibit differential degradation. Some IPLs, especially glycolipids, have the potential to be preserved as fossil molecules for very long time upon dead cells, and therefore, they can not specifically indicate living biomass. Furthermore, the IPLs degradation rate and completeness would be affected by such factors as oxygen concentration and organic matter content. It is also suggested that the composition of IPLs might be affected by microbial metabolism. Therefore, it is essential to take these factors into account when IPLs are used as proxies to trace marine microbial activities and reconstruct the palaeoenvironment.

Key words: Intact polar lipids    Microbial activities    Marine sediment    Suspended particulate matter    Application limitations.
收稿日期: 2015-05-12 出版日期: 2015-10-20
:  P735  
基金资助:

国家重点基础研究发展计划项目“西南印度洋洋中脊热液成矿过程与硫化物矿区预测”(编号:2012CB417300); 中国大洋协会国际海底区域项目“西南印度洋多金属硫化物合同区环境基线综合集成研究”(编号:DY125-11-E-05)资助

通讯作者: 杨群慧(1969-),女,湖南衡阳人,副教授,主要从事海洋生物地球化学研究. E-mail:yangqh@tongji.edu.cn     E-mail: 11_songmin@tongji.edu.cn
作者简介: 宋敏(1989-),女,贵州金沙人,硕士研究生,主要从事海洋生物地球化学研究.
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引用本文:

宋敏, 杨群慧, 王华, 季福武, 王虎, 潘安阳, 周怀阳. 完整极性脂质化合物对海洋微生物活动的指示及应用局限性[J]. 地球科学进展, 2015, 30(10): 1162-1171.

Song Min, Yang Qunhui, Wang Hua, Ji Fuwu, Wang Hu, Pan Anyang, Zhou Huaiyang. Applications of Intact Polar Lipids for Tracing the Marine Microbial Activity and Their Limitations. Advances in Earth Science, 2015, 30(10): 1162-1171.

链接本文:

http://www.adearth.ac.cn/CN/10.11867/j.issn.1001-8166.2015.10.1162        http://www.adearth.ac.cn/CN/Y2015/V30/I10/1162

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