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地球科学进展  2006, Vol. 21 Issue (10): 1058-1062    DOI: 10.11867/j.issn.1001-8166.2006.10.1058
研究论文     
南海西沙海槽沉积物细菌多样性初步研究
李涛,王鹏,汪品先
同济大学海洋地质国家重点实验室,上海 200092
A Preliminary Study on the Diversity of Bacteria in the Xisha Trough Sediment, the South China Sea
Li Tao,Wang Peng,Wang Pinxian
State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, China
 全文: PDF(119 KB)  
摘要:

对来自南海西沙海槽的深海沉积物进行总DNA提取,并构建细菌16S rDNA文库。细菌16S rDNA系统发育分析表明沉积物中细菌分属4个类群:变形杆菌(Proteobacteria)、浮霉菌(Planctomycene)、低G+C含量革兰氏阳性菌和放线菌(Actinobacteria),它们分别占总体的49%、22%、22%和7%。Proteobacteria类群又以δ-Proteobacteria亚群占优势,其他分属α-和γ-Proteobacteria亚群。Planctomycene和低G+C含量革兰氏阳性菌两个常见于浅海的类群,在西沙海槽沉积物的丰度高于其他深海沉积物,可能是随沉积物被浊流搬运至海槽。

关键词: 海洋沉积物16S rDNA基因细菌多样性西沙海槽    
Abstract:

The bulk DNA was isolated from bacterial habits in sediments of the Xisha trough, the south China sea. Using a pair of primers special for most common bacteria, the researchers amplified partial sequences of bacterial 16S rDNA gene and thus constructed a 16S rDNA clone library. A total of 22 clones were used to reconstruct the bacterial 16S rDNA phylogenetic tree. Guided by the phylogenetic tree, bacteria fell into four main lineages: Proteobacteria, Planctomycene, Actinabacteria and Low G+C Gram-positive bacterium. Among them, 49% belonged to Proteobacteria, 22% to Planctomycene,the same to Low G+C Gram-positive bacterium,and the remaining 7% to Planctomycene. The group of Proteobacteria clustered in three subgroups: α-, γ- and δ-Proteobacteria, and the subgroup of δ-Proteobacteria predominated in them. Furthermore, the abundance of two groups of Planctomycene and Low G+C Gram-positive bacterium, living commonly in shallow marine deposits, was higher in sediments of the Xisha Trough than in other deep-sea sediments. Most members of these two groups were probably brought from the shelf into the trough sediment by the turbid.

Key words: Marine sediment    Bacterial diversity.    The Xisha trough    16S rDNA
收稿日期: 2006-02-27 出版日期: 2006-10-15
:  P736.21  
基金资助:

国家自然科学基金项目“南海及相邻海域古海洋学研究”(编号:40510487);海洋地质国家重点实验室开放基金项目资助.

通讯作者: 汪品先(1936-),男,江苏苏州人,中国科学院院士,主要从事海洋地质、微体古生物学研究.     E-mail: pxwang@online.sh.cn
作者简介: 李涛(1980-),男,湖北监利人,博士研究生,主要从事地质微生物学方面研究.E-mail:lukelitao80@163.com
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引用本文:

李涛,王鹏,汪品先. 南海西沙海槽沉积物细菌多样性初步研究[J]. 地球科学进展, 2006, 21(10): 1058-1062.

Li Tao,Wang Peng,Wang Pinxian. A Preliminary Study on the Diversity of Bacteria in the Xisha Trough Sediment, the South China Sea. Advances in Earth Science, 2006, 21(10): 1058-1062.

链接本文:

http://www.adearth.ac.cn/CN/10.11867/j.issn.1001-8166.2006.10.1058        http://www.adearth.ac.cn/CN/Y2006/V21/I10/1058

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