A Review of Techniques of Purification of Source-Specific Sterols for Compound-Specific Isotope Analysis and Applications

doi:10.11867/j.issn.1001-8166.2022.069

Advances in Earth Science ›› 2022, Vol. 37 ›› Issue (12): 1223-1231. doi: 10.11867/j.issn.1001-8166.2022.069

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A Review of Techniques of Purification of Source-Specific Sterols for Compound-Specific Isotope Analysis and Applications

Li LI ¹( ), Meng YU ¹ ^, ²( ), Meixun ZHAO ¹ ^, ²

^1.Frontiers Science Center for Deep Ocean Multispheres and Earth System, and Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China
^2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

Received:2022-05-03 Revised:2022-09-14 Online:2022-12-10 Published:2022-12-16
Contact: Meng YU E-mail:lilee@ouc.edu.cn;yumeng@ouc.edu.cn
About author:LI Li (1982-), female, Pingdu City, Shandong Province, Ph. D student. Research area includes marine organic geochemistry. E-mail: lilee@ouc.edu.cn
Supported by:
the National Natural Science Foundation of China “Burial efficiency and mechanistic controls of terrestrial organic matter in East China Sea: constrained by biomarker and 14C”(41906032)

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Li LI, Meng YU, Meixun ZHAO. A Review of Techniques of Purification of Source-Specific Sterols for Compound-Specific Isotope Analysis and Applications[J]. Advances in Earth Science, 2022, 37(12): 1223-1231.

Source-specific biomarkers are the best targets for isotope analysis and paleoclimatic applications. Compound-specific hydrogen isotope (δ²H) measurements of sterols can be applied to the reconstruction of hydroclimatic conditions and paleo-sea surface salinity. Precise measurements using gas chromatography-isotope ratio mass spectrometry require a high baseline resolution between target analyte and adjacent compounds. However, isotope analysis of sterols for paleoclimatic applications is limited due to the difficulty in purifying complex natural samples. This study aimed to review several improved purification methods of source-specific sterols using liquid chromatography and the applications of compound-specific isotope analysis of sterols in lakes or seas. In addition, we successfully purified dinosterol and brassicasterol from the Chinese marginal sea surface sediments, meeting the requirements for δ²H and carbon isotope analysis. This study provides significant insights and technical support for the paleoenvironmental reconstruction in the Chinese marginal seas.

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