With the socio-economic development, water demand has increased, resulting in river environment flow often not being met. As an important indicator of river ecosystem, environment flow plays a key role in maintaining the sustainability of the ecosystem. This study fully considered the biological needs of river ecosystems, used food web models (Ecopath) to identify key species of fish, determined the ecological flow velocity, combined the river sections, and used the Adapted Ecological Hydraulic Radius Method (AEHRA) to calculate the environment flow. Based on the calculation results of the environment flow, the River2D model was used to simulate the river flow, and the environment flow satisfaction rate of the river was calculated. The calculation results show that the environment flow of each river in the flood season can be satisfied, and the environment flow of most rivers in the non-flood season cannot be satisfied, and the satisfaction rate is extremely low, which causes the ecosystem health to be destroyed. Therefore, ecological regulation of rivers during non-flood seasons should be strengthened to meet river environment flow. The new method for calculating the environment flow of the river provided in this paper can provide a method for aquatic ecological restoration, and the research results can provide important scientific basis for the aquatic ecological restoration and management of rivers in Jinan.