Marine sedimentary basin is an important tectonic unit in the earth, and the evolution of marine sedimentary basin involves a series of the coupling and evolution of geodynamic mechanism such as the crust-mantle, the lithosphere-asthenosphere, the strata-fluid deposition. Therefore, the study of marine sedimentary basin dynamics includes deep structure state of earth, material composition and regional tectonic evolution, and also internal structure, tectonic characteristics and pore fluid characteristics strata of the basin. Wide angle Ocean Bottom Seismometer (OBS) seismic exploration is a marine geophysical survey method originated and developed since 1980’s and 1990’s, which has the advantages of strong penetration capability, high seismic imaging precision and reception of both P-wave and S-wave, and playing an increasing significant role in the research of marine sedimentary basin at the aspect of regional tectonic evolution, internal structure and pore fluid development characteristics of strata in recent years. In the study of passive continental margin, the crustal structure acquired from wide angle OBS seismic data provides the direct evidence that divides the passive continental margins into magma-poor and magma-dominated ones, and the degree of thinning and spatial variation characteristics of crust provide constraints for dynamics simulations of tectonic evolution in marine sedimentary basin. In the study of the structure features of basin, wide angle OBS seismic exploration fills in gaps at the aspect of investigation depth and complex geological structure in conventional multi-channel seismic survey, and acquires overpressure distribution status of basin according to the velocity structure characteristics of strata, and then infer the basin sedimentation velocity and pore fluid characteristics. In the study of internal fluid system in marine sedimentary basin, it reveals the velocity structure of natural gas hydrate reservoir through the analysis and processing of wide angle OBS seismic data, and calculates the thickness of natural gas hydrate reservoir and the content of hydrate and free gas in pore based on the velocity variation. Of course, the future wide angle OBS seismic exploration in the aspect of dynamic evolution and mechanism research in marine sedimentary basin will play a more important role with the development of marine seismic exploration technology, the improvement of data processing and instrument parameters.