How to utilize sulfur isotope for many domestic and foreign researchers to trace the sulfur source of metallic sulfide deposit has been explored for many years. Fruitful results have been gained now. Based on summing up the characteristics of sulfur isotopic composition of hydrothermal mineral from metallic sulfide deposits, this paper illuminated the total sulfur isotopic composition of ore-forming fluids is the key factor in estimating the sulfur source. This paper also summarized three approaches about how to obtain the total sulfur isotopic composition (δ³⁴S_∑S) of ore-forming fluids. They are physical-chemical equilibrium analysis, mineral paragenetic association analysis and Pinckey-Rafter, respectively. We gave some applied examples and made a brief comment of them as well. There are three points worth noting. Firstly, choosing appropriate approach is a critical factor to acquire the δ³⁴S_∑S successfully according to the characteristics of different types of metallic sulfide deposit. Secondly, currently, these above mentioned approaches and applications are effective after the establishment of equilibrium state of sulfur isotope. As to the sulfur isotopic disequilibrium condition in metallic sulfide deposits probably resulted from lower temperature or rapid emplacement, there are quite some problems in theory and technique. Therefore, an in-depth study should also be continued. Thirdly, it is a new development trend to study isotope composition of different forms of sulfur and to discuss their source, forming environment and process respectively, which is probably more effective and significant for tracing sulfur sources of metallic sulfide deposits.