地球科学进展 ›› 2013, Vol. 28 ›› Issue (2): 243 -252. doi: 10.11867/j.issn.1001-8166.2013.02.0243

综述与评述 上一篇    下一篇

海洋溶藻功能菌作用机理研究的若干进展
李 东 1,2, 李 祎 1,郑天凌 1*   
  1. 1.厦门大学生命科学学院,近海海洋环境科学国家重点实验室及滨海湿地生态系统教育部重点实验室,福建 厦门 361102; 2.华侨大学化工学院, 福建 厦门 361021
  • 收稿日期:2012-07-09 修回日期:2012-10-29 出版日期:2013-02-10
  • 通讯作者: 郑天凌 (1955-),男,福建莆田人,教授,主要从事应用与环境微生物研究.E-mail:microzh@xmu.edu.cn E-mail:microzh@xmu.edu.cn
  • 基金资助:

    国家自然科学基金重点项目“我国近海海洋细菌的生态过程及关键菌群影响赤潮生消的机理”(编号: 40930847);国家自然科学基金面上项目“一株海洋高效抑藻活性肽产生菌的研究”(编号: 31070442);高等学校博士学科点专项科研基金优先发展领域“一株新分离的特殊、高效溶藻微生物的作用机理与生态过程研究”(编号:20120121130001)资助.

Advance in the Research of Marine Algicidal Functional Bacteria and Their Algicidal Mechanism

Li Dong 1,2,Li Yi 1,Zheng Tianling 1   

  1. 1.State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems,School of Life Sciences,Xiamen University,Xiamen 361102,China;2.College of Chemical Engineering,Huaqiao University,Xiamen 361021,China
  • Received:2012-07-09 Revised:2012-10-29 Online:2013-02-10 Published:2013-02-10

基于“菌—藻关系”的理念,深入探讨海洋溶藻功能细菌作为潜在的有效防治有害藻华的微生物,已成为学者们的研究热点。但大多的研究主要涉及菌株的分离、鉴定及功能活性物质的纯化,溶藻机理的研究鲜有报道。主要概述了当前国内外海洋环境中溶藻功能细菌溶藻机理的研究进展,并探讨分析了利用海洋溶藻功能细菌防治有害藻华的未来发展方向,提出了该领域今后的重点研究任务。

Based on the relationship between bacteria and algae,the marine algicidal bacteria as a potential microorganisms to effectively eliminate the impact of harmful algae blooms,has become the research hot topic.But most researches are  mainly involves in the algicidal bacteria isolation,identification and the algicidal substances purification.However,the mechanisms of bacteria lysing algae are rarely reported.Here,the latest development of algicidal mechanisms is briefly reviewed,and the future development prospects and trends are also discussed.

中图分类号: 

[1]Zhou Mingjiang, Zhu Mingyuan.Progress of the project“ecologyand oceanography of harmful algal blooms in China”[J]. Advances in Earth Science,2006,21 (7):673-679. [周名江,朱明远.“我国近海有害赤潮发生的生态学、海洋学机制及预测防治”研究进展[J]. 地球科学进展,2006,21(7):673-679.]



[2]Anderson D M.Approaches to monitoring,control and management of Harmful Algal Blooms (HABs) [J].Ocean & Coastal Management,2009,52(7):342-347.



[3]Wu Qinglong, Xie Ping,Yang Liuyan,et al.Ecological consequences of cyanobacetrial blooms in lakes and their countermeasures [J].Advances in Earth Science,2008,23 (11):1 115-1 123.[吴庆龙,谢平,杨柳燕,等.湖泊蓝藻水华生态灾害形成机理及防治的基础研究[J]. 地球科学进展,2008,23(11):1 115-1 123.]



[4]Imai I,Kim M C,Nagasaki K,et al.Relationships between dynamics of red tide-causing raphidophycean flagellates and algicidal micro-organisms in the coastal sea of Japan[J].Phycological Research,1998,46(2):139-146.



[5]Oh J I,Kim M J,Lee J Y,et al.Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture[J]. Biotechnology and Bioprocess Engineering,2011,16(6):1 124-1 133.



[6]Chen W M,Sheu F S,Sheu S Y.Aquimarina salinaria sp.nov.,a novel  algicidal bacterium isolated from a saltpan[J].Archives Microbiology,2012,194(2):103-112.



[7]Kim H S,Ahn C Y,Joung S H,et al.Growth inhibition of Microcystis aeruginosa by a glycolipid-type compound from Bacillus subtilis C1[J].Journal of Microbiol Biotechnol,2010,20(8):1 240-1 242.



[8]Wang B X,Zhou Y Y,Bai S J,et al.A novel marine bacterium algicidal to the toxic dinoflagellate Alexandrium tamarense[J].Letters in Applied Microbiology,2010,51(5):552-557.



[9]Sakata T,Yoshikawa T,Nishitarumizu S.Algicidal activity and identification of an algicidal substance produced by marine Pseudomonas sp.C55a-2[J].Fisheries Science,2011,77(3):397-402.



[10]Zhao Yijun,Liu Yongding.Possible microbial control on the adverse impacts of algae-current information about the relationship between algae and microbes[J].Acta Hydrobilolgica Sinica,1996,20(2):173-181.[赵以军,刘永定.有害藻类及其微生物防治的基础——藻菌关系的研究动态[J].水生生物学报,1996,20(2):173-181.]



[11]Shilo M.Lysis of blue-green algae by myxobacter[J].Journal of  Bacteriology,1970,104(1):453-461.



[12]Mitsutani A,Takesue K,Kirita M,et al.Lysis of Skeletonema costatum by Cytophaga sp.isolated from the coastal water of the Ariake Sea[J].Nippon Suisan Gakkaishi,1992,58(11):2 159-2 169.



[13]Imai I,Ishida Y,Hata Y.Killing of marine phytoplankton by a gliding bacterium Cytophaga sp.,isolated from the coastal sea of Japan[J].Marine Biology,1993,116(4):527-532.



[14]Su J Q,Yang X R,Zheng T L,et al.Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense[J].Harmful Algae,2007,6(6):799-810.



[15]Mayali X,Azam F.Algicidal bacteria in the sea and their impact on algal blooms[J].Journal of Eukaryotic Microbiology,2004,51(2):139-144.



[16]Bai S J,Huang L P,Su J Q,et al.Algicidal effects of a novel marine actinomycete on the toxic dinoflagellate Alexandrium tamarense[J].Current Microbiology,2011,62(6):1 774-1 781.



[17]Fu Lijun, An Xinli,Zheng Tianling.Advances in algicidal substances produced by algae-iysing actinomycetes[J].Advances in Earth Science,2010,25(9):960-965.[傅丽君,安新丽,郑天凌.环境中放线菌及其抑藻活性物质研究的若干进展[J]. 地球科学进展,2010,25(9):960-965.]



[18]Roth P B,Twiner M J,Mikulski C M,et al.Comparative analysis of two algicidal bacteria active against the red tide dinoflagellate Karenia brevis[J].Harmful Algae,2008,7(5):682-691.



[19]Qu Jianhong,Liu Shaobin.The growth of Bacillus sp and Microcystis  aeruginosa and their competition for resources[J].Journal of Zhanjiang Ocean University,2002,22(3):13-18.[瞿建宏,刘韶斌.水体中芽孢杆菌和微囊藻的生长及其资源竞争[J].湛江海洋大学学报,2002,22(3):13-18.]



[20]Furusawa G,Yoshikawa T,Yasuda A,et al.Algicidal activity and gliding motility of Saprospira sp.SS98-5[J].Canadian Journal of Microbiology,2003,49(2):92-100.



[21]Kirchman D L.The ecology of Cytophaga-Flavobacteria in aquatic environments[J].FEMS Microbiology Ecology,2002,39(2):91-100.



[22]Caiola M G,Pellegrini S.Lysis of microcystis aeruginosa (KTZ) by bdellovibrio-like bacterial[J].Journal of Phycology,1984,20(4):471-475.



[23]Wang Fei, Li Yan. Development progress of the separation of extracellular algae-lysing components from algae-lysing bacteria[J].Journal of Anhui Agricultural Sciences,2008,36(17):7 071-7 073.[王飞,李燕.溶藻细菌胞外溶藻活性物质分离的研究进展[J].安徽农业科学,2008,36(17):7 071-7 073.]



[24]Wang B X,Yang X R,Yanyan Z,et al.An algicidal protein produced by bacterium isolated from the Donghai Sea,China[J].Harmful Algae,2012,13:83-88.



[25]Jeong S Y,Ishida K,Ito Y,et al.Bacillamide,a novel algicide from the marine bacterium,Bacillus sp.SY-1,against the harmful dinoflagellate,Cochlodinium polykrikoides[J].Tetrahedron letters,2003,44(43):8 005-8 007.



[26]Yoshikawa K,Adachi K,Nishijima M,et al.β-Cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacteria[J].Applied and Environmental Microbiology,2000,66(2):718-722.



[27]Kawano Y,Nagawa Y,Nakanishi H,et al.Production of thiotropocin by a marine bacterium, Caulobacter sp.and its antimicroalgal activities[J].Journal of Marine Biotechnology,1997,5:225-229.



[28]Paul C,Pohnert G.Interactions of the algicidal bacterium kordia algicida with Diatoms:Regulated protease excretion for specific algal lysis[J].PLoS One,2011,6(6):e21032.



[29]Su J Q,Yang X R,Zhou Y Y,et al.Marine bacteria antagonistic to the harmful algal bloom species Alexandrium tamarense (Dinophyceae)[J].Biological Control,2011,56(2):132-138.



[30]Jeong H,Yim J H,Lee C,et al. Genomic blueprint of Hahella chejuensis,a marine microbe producing an algicidal agent[J].Nucleic Acids Research,2005,33(22):7 066-7 073.



[31]Kwon S K,Park Y K,Kim J F.Genome-wide screening and  identification of factors affecting the biosynthesis of prodigiosin by Hahella chejuensis,using Escherichia coli as a surrogate host[J].Applied and Environmental Microbiology,2010,76(5):1 661-1 668.



[32]Nakashima T,Miyazaki Y,Matsuyama Y,et al.Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium gamma-proteobacterium[J].Applied Microbiol Biotechnol,2006,73(3):684-690.



[33]Wang X L,Gong L Y,Liang S K,et al.Algicidal activity of  rhamnolipid biosurfactants produced by Pseudomonas aeruginosa[J].Harmful Algae,2005,4(2):433-443.



[34]Kodani S,Imoto A,Mitsutani A,et al.Isolation and identification of the antialgal compound,harmane (1-methyl-β-carboline),produced by the algicidal bacterium,Pseudomonas sp.K44-1[J].Journal of Applied Phycology,2002,14(2):109-114.



[35]Banin E,Khare S K,Naider F,et al.Proline-rich peptide from the coral pathogen vibrio shiloi that inhibits photosynthesis of Zooxanthellae[J].Applied and Environmental Microbiology,2001,67(4):1 536-1 541.



[36]Nagasaki K,Yamaguchi M,Imai I.Algicidal ativity of a killer bacterium against the harmful red tide dinoflagellate Heterocapsa circularisquama isolated from Ago Bay,Japan[J].Nippon Suisan Gakkaishi,2000,66(4):666-673.



[37]Shunyu S,Yongding L,Yinwu S,et al.Lysis of Aphanizomen on flos-aquae (Cyanobacterium) by a bacterium Bacillus cereus[J].Biological Control,2006,39(3):345-351.



[38]Yang Xiaoru,Su Jianqiang,Zheng Tianling.The importance and potential application of allelopathy in red-tide control[J].Acta Scientiae Circumstantiae,2008,28(2):219-226.[杨小茹,苏建强,郑天凌.化感作用在赤潮调控中的意义及前景[J].环境科学学报,2008,28(2):219-226.]



[39]Zhang Bin,Guo Jinsong,Fang Fang,et al.Action mechanisms of allelopathy in inhibiting algae:A review[J].Chinese Journal of Ecology,2010,(9):1 846-1 851.[张彬,郭劲松,方芳,等.植物化感抑藻的作用机理[J].生态学杂志,2010,(9):1 846-1 851.]



[40]Yang Lili,Pan Weibin,Li Yan.Preliminary study on components and its algicidal mechanism of algicidal substance secreted by bacteria[J].Environmental Science & Technology,2010,33(3):72-75.[杨丽丽,潘伟斌,李燕.一株溶藻细菌溶藻活性物质的成份及溶藻机制[J].环境科学与技术,2010,33(3):72-75.]



[41]Sun X X,Choi J K,Kim E K.A preliminary study on the mechanism of harmful algal bloom mitigation by use of sophorolipid treatment[J].Journal of Experimental Marine Biology and Ecology,2004,304(1):35-49.



[42]Ozaki K,Ito E,Tanabe S,et al.Electron microscopic study on lysis of a cyanobacterium Microcystis[J].Journal of Health Science,2009,55(4):578-585.



[43]Wang Xin,Zhou Lihong,Zheng Tianling,et al.Lysis of Alexandrium Tamarense mediated by bacteria in its phycosphere[J].Acta Ecologica Sinica,2007,27(7):2 864-2 871.[王新,周立红,郑天凌,等.塔玛亚历山大藻藻际细菌溶藻过程[J].生态学报,2007,27(7):2 864-2 871.]



[44]Wang L,Zheng B.Toxic effects of fluoranthene and copper on marine diatom Phaeodactylum tricornutum[J].Journal of Environmental Sciences,2008,20(11):1 363-1 372.



[45]Huang Changmiao,Pan Weibin,Li Yan,et al.Effect of three algicidal bacteria strains on antioxidase activities of anabaena flos-aquae[J].Environmental Science and Technology,2009,(6):10-13.[黄昌妙,潘伟斌,李燕,等.三株溶藻菌对水华鱼腥藻抗氧化酶活性的影响[J].环境科技,2009,(6):10-13.]



[46]Wang Yibin,He Bijuan,Zheng Zhou,et al.Preliminary study on  identification of algicidal active substance from mangrove bacteria Flavobacterium sp.and its algicidal effect on red tide algae Alexandrium tamarense[J].Chinese Journal of Marine Drugs,2008,27(6):1-4.[王以斌,何碧娟, 郑洲,等.红树林细菌 Flavobacterium sp.的抑藻活性物质鉴定及其对亚历山大藻抑制作用的初步研究[J].中国海洋药物,2008,27(6):1-4.]



[47]Wang Q,Su M,Zhu W,et al.Growth inhibition of Microcystis aeruginosa  by white-rot fungus Lopharia spadicea[J].Water Science and Technology,2010,62(2):317-323.



[48]Shi S,Tang D,Liu Y.Effects of an algicidal bacterium Pseudomonas  mendocina on the growth and antioxidant system of Aphanizomenon  flos-aquae[J].Current Microbiology,2009,59(2):107-112.



[49]Fu Lijun,Li Dong,Wu Chengji,et al.Effects of algicidal bacterium BS03(Microbulbifer sp.) on the growth and antioxidant systems of Alexandrium tamarense[J].Acta Microbiologica Sinica,2012,52(6):784-790.[傅丽君,李东,吴承集,等.溶藻细菌 BS03 (Microbulbifer sp.) 对塔玛亚历山大藻生长及抗氧化系统的影响[J].微生物学报,2012,52(6):784-790.]



[50]Hibayashi R,Imamura N.Action mechanism of a selective anti-cyanobacterial compound,argimicin A[J].Journal of Antibiotics,2003,56(2):154-159.



[51]Nishiyama Y,Yamamoto H,Allakhverdiev S I,et al.Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery[J].EMBO Journal,2001,20(20):5 587-5 594.



[52]Hong Y,Hu H Y,Xie X,et al.Gramine-induced growth inhibition, oxidative damage and antioxidant responses in freshwater cyanobacterium Microcystis aeruginosa[J].Aquatic Toxicology,2009, 91(3):262-269.



[53]Leu E,Krieger-Liszkay A,Goussias C,et al.Polyphenolic allelochemicals from the aquatic angiosperm Myriophyllum Spicatum Inhibit Photosystem II[J].Plant Physiology,2002,130(4):2 011-2 018.



[54]Shao J,Wu Z,Yu G,et al.Allelopathic mechanism of pyrogallol to Microcystis aeruginosa  PCC7806 (Cyanobacteria):From views of gene Expression and antioxidant system[J].Chemosphere,2009,75(7):924-928.



[55]Ishio S,Mangidaan R,Kuwahara M,et al.A bacterium hostile to flagellates:Identification of species and characters[C]∥Okaichi T,Anderson D M,Nemoto T, eds.Red Tides. New York:Elsevier,1989:205-208.



[56]Li Shoutian, Zhou Jianmin,Wang Huoyan,et al.Allelopathic mechanism of plants[J].Rural Eco-environment,2001,17(4):52-55.[李寿田,周健民,王火焰,等.植物化感作用机理的研究进展[J].农村生态环境,2001,17(4):52-55.]



[57]Deng Jianming,Tao Yong,Li Daping,et al.Advances in research of algicidal bacteria[J].Chinese Journal of Applied & Environmental Biology,2009,15(6):895-900.[邓建明,陶勇,李大平,等.溶藻细菌及其分子生物学研究进展[J].应用与环境生物学报,2009,15(6):895-900.]



[58]Xu Y,Ge F,Wang N,et al.Selective algicidal activity of surfactant and its mechanism[J].Journal of Hazardous,Toxic,and Radioactive WasteManagement,2011,15(1):21-25.



[59]Ozaki K,Ohta A,Iwata C,et al.Lysis of cyanobacteria with volatile organic compounds[J].Chemosphere,2008,71(8):1 531-1 538.



[60]Tang Shuishui,Lin Weitie,Li Jingyuan,et al.Isolation and algicidal characteristics of the algicidal components from actinomycete strain L74[J].Microbiology China,2011,38(5):654-659.[唐水水, 林炜铁,李敬源,等.放线菌 L74 溶藻物质的分离及其溶藻特性[J].微生物学通报,2011,38(5):654-659.]



[61]Lee H S,Kang S G,Kwon K K,et al.Genome sequence of the algicidal bacterium kordia algicida OT-1[J].Journal of Bacteriology,2011,193(15):4 031-4 032.



[62]Mahong B,Roytrakul S,Phaonaklop N,et al.Proteomic analysis of a model unicellular green alga,Chlamydomonas reinhardtii, during short-term exposure to irradiance stress reveals significant down regulation of several heat-shock proteins[J].Planta,2012,235(3):499-511.



[63]Yuan Yawei,Zheng Wei,Li Shaojing,et al.Comparison of three protein extraction methods for proteomic analysis of Alexandrium tamarense with two-dimensional electrophoresis[J].Acta Microbiologica Sinica,2011,51(8):1 113-1 118.[原雅纬,郑伟,李少菁,等.塔玛亚历山大藻双向电泳蛋白的三种提取方法比较[J].微生物学报,2011,51(8):1 113-1 118.]



[64]Affenzeller M J,Darehshouri A,Andosch A,et al. Salt stress-induced cell death in the unicellular green alga Micrasterias denticulata[J].Phytion-international Journal of Exprimental Botany,2009,60(3):939-954.



[65]Darehshouri A, Affenzeller M,Lütz-Meindl U.Cell death upon H2O2 induction in the unicellular green alga Micrasterias[J].Plant Biology,2008,10(6):732-745.

[1] 王丹,刘桂梅,何恩业,李 津. 有害藻华的预测技术和防灾减灾对策研究进展[J]. 地球科学进展, 2013, 28(2): 233-242.
阅读次数
全文


摘要