Lakes play an essential role in the evolution of regional water cycles and ecosystems. In previous studies on lake evolution, most lake sediment proxy indicators have been used to reconstruct lake and climate change processes. However, there is a lack of quantitative research on the lake water cycle characteristics. Based on the water balance model for watersheds and lakes in distinct periods and the lake energy balance model based on the simulation of the transient climate, water balance calculations and lake evolution simulations for six typical lakes in the Qinghai-Tibet Plateau and its surrounding areas were carried out in this study. The results showed that the precipitation and evaporation variabilities in Xiao Qaidam Lake and Lop Nur were relatively small during the Holocene. The precipitation and evaporation variabilities in Selinco and Namco were relatively large during the early-middle Holocene, mainly controlled by temperature and net radiation changes. The precipitation and evaporation variabilities in Qinghai Lake and Zhuyeze were close during the early and mid-late Holocene. This study systematically analyzed and calculated the evolution of lake water cycle elements in different climatic regions of the Qinghai-Tibet Plateau during the Holocene, which will help to understand the paleoclimatic mechanism of lake evolution in this region.