The agricultural development of mountainous areas in China is undergoing the transformation from the traditional agricultural land use mode dominated by single and inefficient grain production to the new agricultural land use mode such as high-yield and efficient horticultural crops. The research on the impact of farmland fragmentation on the agricultural land intensity degree is the basis of agricultural modernization and land remediation in mountainous areas. Based on the UAV high-resolution remote sensing image data of two typical villages in Guizhou Province and the questionnaire survey data of farmers, using the road accessibility index of farmers' plot, the area index and the shape index to measure the degree of farmland fragmentation, we analyzed the impact of different degrees of farmland fragmentation on the traditional and new types of farmland use intensification. The research showed that the farmland fragmentation had a significant negative impact on the intensive degree of agricultural land, but the impact on the intensive degree of agricultural land in different ways of use was different. With the intensification of the degree of farmland fragmentation, the impact on the input and output of horticultural crops and other new-type agricultural land utilization was less than that of grain crops and other traditional agricultural land utilization. Adjusting agricultural structure and transformation of farmland utilization greatly alleviated the negative impact of farmland fragmentation on farmland intensity in mountainous areas.