On the basis of our regional geological survey report of Chengde Basin on the scale of 1∶ 50000, it was the first time to discover the stromatolites in Yeli-Formation with stratigraphic determination techniques and isotope analysis techniques. It is well known that Yeli Formation is consisted of gray-green thin limestone with yellowish-green and gray-black shale. The bottom of Yeli Formation is thick black limestone with pyrite nodules. According to the characteristics of lithology, structure, sedimentary structure and microscopic microfacies, Yeli Formation can be divided into shale facies and limestone facies. Yeli Formation can be identified into six kinds of basic sequence according to the limestone assemblage, lithological facies characteristics and phase sequence rule. The ingredient of these stromatolites was recognized as Jacutophyton, Colloumnacollenia, Katavia and Paraconophyton. The discovery of stromatolites as shown in Chengde Basin amplitude regional geological survey report has filled the gap in the biogenic strata of the Yeli Formation. Besides, the contents of Ordovician biostragraphy have been enriched according to our regional geological survey of Chengde Basin. The growing environment of stromatolites was mainly in the lower tidal zone with the lower energy environment, sometime transformed the upper tidal zone with the upper energy environment. The growing environment of stromatolites can be concluded through the morphology of stromatolites. From the morphology of stromatolites, it also revealed that Ordovician biostragraphy belongs to vice-sequence stratum deposition mode from bottom up, deep and shallow. Combination of lithological facies and basic sequence analysis, the overall phase sequence characteristics of Yeli-Formation can be described as follows: Shales in shelf facies→glauconite sandstone in ramp facies→sparite spergenite, lump sparite, laminated limestone in subtidal-intertidal belt→dolomitization-micrite in intertical belt. The shallowing upwards sedimentary sequence was formation ultimately. According to the shallowing upwards sedimentary sequence, it could be deduced that the environment of Yeli Formations was from shallow sea shelf to intertidal environment by the Shallow sea shelf to intertidal environment. This conclusion was in good agreement with the conclusion of its sedimentary environment based on the lithologic facies and the basic sequence of the Yeli Formation. Our regional geological survey report of Chengde Basin provides a new understanding of stromatolites.