By EOF, Power spectrum and nonlinear mapping methods, the temperature time series of May to July in Jianghuai river basins from 1948-2014 (67 years) were objectively divided into several climate stages. The time series were divided into three climate stages with similar lengths: the stage with its air temperature trend going downwards stage (1948-1970); the stage with its air temperature trend going upwards (1971-1994) and the stage with its air temperature trend going gentle upwards (1995-2014). Their climate characteristics can be quantified by a special climate index. Then, the statistic characteristics of Jianghuai cyclones in the three climate stages were compared with the cooling or warming trends. The results showed that characteristic evolutions of the frequency of yearly average of Jianhuai cyclones, the cyclones with their life cycles in the area longer than 48h, the cyclones with three types of thickness, shallow and bottom shapes classified according to the cyclone height, the cyclones with three kinds of zonal, meridional, and circular trajectory, responded to the temperature changing trends in the three climate stages. The cooling trends of the stage temperature restrained the activity of Jianghuai cyclones, and the warming trends of the stage temperature promoted the cyclone’s development, maintaining, activity, and also led cyclone moving towards to north further. The composite analysis of cyclone structures showed that the evolution characteristics in the center intensity, temperature grads, the maxima velocity of jet of the cyclones, and the intensity, length and position of the vapor flux passage of the Jianghuai cyclones, also the intensity, thickness, the positions of the warm and cold air masses within the cyclones, all responded to the trend change of stage temperature including cooling, warming and gentle warming. The results show clearly that temperature cooling reduces the intensity of cyclone structures and restrains the activity of cyclones in the temperatures trend down stage. The temperature warming enhances the stable development and strengthening of cyclone structure,and maintains the activity of cyclones in the temperature warming stages.