Carbon dioxide is a common and important component in fluid inclusions. Because carbon isotopic ratio of CO₂ in fluid inclusion can provide geochemical characteristics of source rocks, many works have been conducted to measure the carbon isotopic ratio (¹³C/¹²C) of CO₂. In general, carbon isotopic composition (¹³C/¹²C) of CO₂ fluid inclusions are measured by mass spectroscopy. However, mass spectroscopy is a destructive analytical technique, and can not be applied to measure single inclusion. Raman spectroscopy is an efficient non-contact and non-destructive method, and has been widely employed in many research fields. In fact, due to the difference of carbon atom mass, the Femi resonance of ¹³CO₂ is lower than that of ¹²CO₂, so they can be identified in Raman spectrum. In principle, the carbon isotopic composition (¹³CO₂/¹²CO₂) in CO₂ is closely related to the Raman intensity ratio between ¹³CO₂ and ¹²CO₂ ( \begin{array}{c}{I}_{+}^{13}\end{array} / \begin{array}{c}{I}_{+}^{12}\end{array} ). Therefore, Raman spectroscopy can theoretically be utilized to measure the carbon isotopic composition (¹³CO₂/¹²CO₂) of CO₂. However, this method is seriously hampered by the poor measurement accuracy and precision. In this study, based on the theoretical analysis, and in combination with previous Raman studies on carbon isotopic composition in CO₂, a preliminary research on Raman quantitative measurement for carbon isotopic composition in CO₂ was carried out.