Synthetic oil（hydrocarbon）-bearing inclusion were synthesized in Iceland crystal with different Oil/water ratios (3/7，5/5，7/3，9/1), which provided better means for researching the mechanism of trapping oil（hydrocarbon）-bearing inclusion in carbonate. Through microscopic observation and micro-fluorometry, it could be seen that the inclusions distributed along the microcrack, which demonstrated that inclusions were trapped during the process of crack healing. Oil and brine inclusions not only distributed in the same crack, but also in different zone. The quantity of synthetic oil（hydrocarbon）-bearing inclusions increased company with the O/W ratios rising.The fluorescence characteristic of oil inclusions and oil adopted in experiment was coincident. There were no apparent differences among the homogenization temperature of inclusions from different sample. Therefore the hydrocarbon saturation was not the main influential factor of homogenization temperature. Homogenization temperature was affected by pressure and gaseous and liquid phase composition. It could be concluded that crystal growth and inclusions trapping were inhibited by oil and gas, but not terminated. The critic time of hydrocarbon accumulation coincided with trapping much oil inclusions. Zonation of oil and brine inclusions distribution was caused by immiscibility effect between oil and water.