The environment and ecological research, which develops from quality and quantity to comprehensive integrated research of the common participation of multi-subjected, multi-scaled, much-timed groups, needs a comprehensive integrated research platform with strong support ability. The gradually perfected computers, networks, data, model and modeling technologies provide technology and research base for the supportive platform e-science building of environmental and ecological integrative research. By analyzing the basic work process of environmental and ecological integration research model which is on the basis of enormous accumulations of on-the-spot observation and experimental data, and mathematic model pattern, and calculation software, we draw a conclusion that the e-science adapted to the environmental and ecological research is made up of data platform, model and modeling platform, cooperation and communication platform, and management platform. Important research contents include: (1) The collection of fixed field stations original data, collection of internet data, generation of data's reanalysis, collection of sensor network data, collection and processing of spatial data,etc. are the base of e-science.(2) The platform include model repository adapting to ecology and environment, mode repository, cooperation modeling system, simulation system, software development system, etc. are the core of the integrated research supported by e-science. (3) The system of model cooperate with mode and data, development and integration system of the data interface and interface drive among perfected model, mode and data in data platform, and the realization of interior cooperative working platform of modeling and simulated calculation are the core in the e-science application. (4) Improved result shows that outputs and management platform of resource dispatch are e-science's windows. Taking ecological economy, hydrology and the setting of natural protection region for example, we did preliminary experiments of e-science's construction concerning the above four aspects and came to the conclusion that the e-science framework of the above four-tiers construction in general meets the demands in the environmental and ecological research process, and that it is feasible and operational in the environmental and ecological comprehensive integrated research.