收稿日期: 2009-12-14
修回日期: 2009-12-30
网络出版日期: 2010-02-10
基金资助
国家自然科学基金重点项目“南海冷泉区甲烷通量及其对海底环境与生态系统影响的生物地球化学研究”（编号：40730844）；国家“十一五”大洋专项基金项目“深部生物圈微生物活动在沉积物和间隙水中的地球化学记录研究”（编号：DYXM-115-02-2-17）；国家高技术研究发展计划重点课题“大洋钻探站位调查关键技术研究”（编号：2008AA093001）资助.
A Preliminary Study of Microbial Diversity of the Top Sediment from the MD06-3047
Received date: 2009-12-14
Revised date: 2009-12-30
Online published: 2010-02-10
从黑潮源区采集上层沉积物，进行DNA提取，以细菌和古菌的16S rDNA通用引物PCR扩增黑潮源区沉积物中细菌和古菌群落的16S rDNA，并构建细菌和古菌的16S rDNA 文库，经限制性片段长度多态性分析（Restriction Fragment Length Polymorphism, RFLP），DNA序列测定和系统发育分析，对黑潮源区表层沉积物的细菌和古菌多样性进行了研究。研究结果表明：黑潮源区细菌包括了变形杆菌(Proteobacteria)、酸杆菌(Acidbacterium)、浮霉菌(Planctanycene)、疣微菌(Verrucomicrobia)和Candidate division OP8和拟杆菌(Bacteroidetes)共6个类群, 其中变形杆菌是优势类群。古菌包括了泉古菌(Crenarchaeota)和广古菌(Euryarchaeota)，其中泉古菌占优势；泉古菌包括MCG、C3、MBGA 和MGI 4个类群，而广古菌包括SAGMEG、MBGE和MEG 3个类群，其中MBGE是优势类群。
魏玉利 . 黑潮源区沉积物微生物多样性初步研究[J]. 地球科学进展, 2010 , 25(2) : 212 -219 . DOI: 10.11867/j.issn.1001-8166.2010.02.0212
Total DNA was extracted from the top sediment layer (0-10 cm) of MD06-3047 in the Kuroshio and was used as template for polymerase chain reaction (PCR) amplification employing specific primers for bacterial and archaeal 16S rRNA genes. The 16S rDNA libraries were then constructed. Microbial diversity was analyzed by using restriction fragment length polymorphism (RFLP), DNA sequencing and phylogenetic analysis. The results show that there are six phyla in the Bacteria domain: Proteobacteria, Acidbacterium, Planctanycenea, Verrucomicrobia, Candidate division OP8 and Bacteroidetes, and the phylum Proteobacteria is predominant. In the Archaea domain, Crenarchaeota dominates over Euryarchaeota. There are four divisions in the Crenarchaeota kingdom: MCG, C3, Marine Benthic Group A, Marine Group; three divisions in Euryarchaeota kingdom: South Africa Golden mine Euryarchaeota Group、Marine Benthic Group E and MEG, and the Marine Benthic Group E is the dominating group.
Key words： Kuroshio; 16S rDNA; Archaea; Bacteria; Microbial diversity
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