收稿日期: 2006-02-27
修回日期: 2006-08-20
网络出版日期: 2006-10-15
基金资助
国家自然科学基金项目“南海及相邻海域古海洋学研究”（编号：40510487）；海洋地质国家重点实验室开放基金项目资助.
A Preliminary Study on the Diversity of Bacteria in the Xisha Trough Sediment, the South China Sea
Received date: 2006-02-27
Revised date: 2006-08-20
Online published: 2006-10-15
对来自南海西沙海槽的深海沉积物进行总DNA提取，并构建细菌16S rDNA文库。细菌16S rDNA系统发育分析表明沉积物中细菌分属4个类群：变形杆菌(Proteobacteria)、浮霉菌(Planctomycene)、低G+C含量革兰氏阳性菌和放线菌(Actinobacteria)，它们分别占总体的49%、22%、22%和7%。Proteobacteria类群又以δ-Proteobacteria亚群占优势，其他分属α-和γ-Proteobacteria亚群。Planctomycene和低G+C含量革兰氏阳性菌两个常见于浅海的类群，在西沙海槽沉积物的丰度高于其他深海沉积物，可能是随沉积物被浊流搬运至海槽。
关键词： 海洋沉积物; 16S rDNA基因; 细菌多样性; 西沙海槽
汪品先 , 李涛 , 王鹏 . 南海西沙海槽沉积物细菌多样性初步研究[J]. 地球科学进展, 2006 , 21(10) : 1058 -1062 . DOI: 10.11867/j.issn.1001-8166.2006.10.1058
The bulk DNA was isolated from bacterial habits in sediments of the Xisha trough, the south China sea. Using a pair of primers special for most common bacteria, the researchers amplified partial sequences of bacterial 16S rDNA gene and thus constructed a 16S rDNA clone library. A total of 22 clones were used to reconstruct the bacterial 16S rDNA phylogenetic tree. Guided by the phylogenetic tree, bacteria fell into four main lineages: Proteobacteria, Planctomycene, Actinabacteria and Low G+C Gram-positive bacterium. Among them, 49% belonged to Proteobacteria, 22% to Planctomycene,the same to Low G+C Gram-positive bacterium,and the remaining 7% to Planctomycene. The group of Proteobacteria clustered in three subgroups: α-, γ- and δ-Proteobacteria, and the subgroup of δ-Proteobacteria predominated in them. Furthermore, the abundance of two groups of Planctomycene and Low G+C Gram-positive bacterium, living commonly in shallow marine deposits, was higher in sediments of the Xisha Trough than in other deep-sea sediments. Most members of these two groups were probably brought from the shelf into the trough sediment by the turbid.
Key words： Marine sediment; Bacterial diversity.; The Xisha trough; 16S rDNA
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