收稿日期: 2005-10-31
修回日期: 2006-05-15
网络出版日期: 2006-07-15
基金资助
国家自然科学基金创新群体项目“南海生物地球化学过程与机制”（编号：40521003）；国家自然科学基金项目“特征光合色素对海洋初级生产过程的指示作用研究”（编号：40331004）；国家重点基础研究发展计划项目“我国近海生态系统食物产出的关键过程及其可持续机理”（编号：2006CB400604）资助.
Phytoplankton Community Structure in the Transects across East China Sea and Northern South China Sea Determined by Analysis of HPLC Photosynthetic Pigment Signatures
Received date: 2005-10-31
Revised date: 2006-05-15
Online published: 2006-07-15
刘媛 , 陈纪新 , 洪华生 , 曹振锐 , 黄邦钦 . 应用特征光合色素研究东海和南海北部浮游植物的群落结构[J]. 地球科学进展, 2006 , 21(7) : 738 -746 . DOI: 10.11867/j.issn.1001-8166.2006.07.0738
Phytoplankton samples from typical stations along the East China Sea and the northern south China sea were analyzed by RP-HPLC combined with DAD (diode array detector) to determine pigments composition. Ocean study included PN section along East China sea(September in 2002) and northern of south China sea(November in 2002). Pigments data were transformed into phytoplankton composition by software CHEMTAX based on least square method and steepest descent algorithm. This work showed that phytoplankton communities in PN transect were clustered into two types, mostly influenced by stratification. Communites in upper layer were dominated by cyanobacteria, while diatoms were most important groups in lower layer. Also two types of phytoplankton community structure in S transect were clustered by the distance from the shore. Diatoms, Cryptophyta, Chlorophyta were main groups in shore, and Prymnesiophyta, Cyanobacteria, Prochlorophyta were main groups offshore. Additionally,primary analysis was carried out to illuminate the relationship of phytoplankton community structure and environmental factors.
[1] Simon N, Barlow R G, Marie D, et al. Characterization of oceanic photosynthetic picoeukaryotes by flow cytometry[J]. Journal of Phycology,1994,30:922-935.
[2] Barlow R G, Mantoura R F C, Gough M A, et al. Pigment signatures of the phytoplankton composition in the northeastern Atlantic during the 1990 spring bloom[J]. Deep-Sea Research II,1993,40:459-477.
[3] Wright S W, Thomas D P, Marchant H J, et al. Analysis of phytoplankton of the Australian sector of the Southern Ocean: Comparisons of microscopy and size frequency data with interpretations of pigment HPLC data using the CHEMTAX' matrix factorisation program[J]. Marine Ecology Progress Series,1996,144:285-298.
[4] Andersen R A, Bidigare R R, Keller M D, et al. A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific Oceans[J]. Deep-Sea Research II,1996,43:517-537.
[5] Gieskes W W C, Kraay G W. Dominance of Cryptophyceae during the phytoplankton spring bloom in the central North Sea detected by HPLC analysis of pigments[J]. Marine Biology,1983,75:179-185.
[6] Letelier R M, Bidigare R R, Hebel D V, et al. Temporal variability of phytoplankton community structure based on pigment analysis[J]. Limnology and Oceanography,1993,38:1 420-1 437.
[7] Laurence D-S, Georges B, Bertrand Le. A chemotaxonomic method to quantify phytoplankton groups in freshwater lentic mesocosms: An approach including chlorophyll a breakdown products[J]. C. R. Biologies,2003,326:95-105.
[8] Mackey M D, Mackey D J, Higgins H W, et al. CHEMTAX-A program for estimating class abundances from chemical markers: Application to HPLC measurements of phytoplankton[J]. Marine Ecology Progress Series, 1996, 144:265-283.
[9] Furuya K, Masato H, Yasushi Y, et al. Phytoplankton dynamics in the East China Sea in spring and summer as revealed by HPLC-derived pigment signatures[J]. Deep-Sea Research II, 2003, 50(3):367-387.
[10] Walsh J J, Wirick C D, Pietrafesa L J, et al. High-frequency sampling of the 1984 spring bloom within the Mid-Atlantic Bight: Synoptic shipboard, aircraft and in situ perspectives of the SEEP-I experiment[J]. Continental Shelf Research,1988,8:529-563.
[11] Jing Zhang. Biogeochemistry of chinese estuarine and coastal waters: Nutrients, trace metals and biomarkers[J]. Region Environmental Change,2002, 3:65-76.
[12] Gu Xingen, Yuan Qi, Yang Jiaowen, et al. Study on vertical distribution of phytoplankton in the water of changjiang estuang[J]. Journal of Fishery Sciences of China,1995,2(1):28-38.[顾新根,袁骐,杨蕉文,等.长江口外水域浮游植物垂直分布研究[J].中国水产科学,1995,2(1): 28-38.]
[13] Hsiu-Ping Li, Gwo-Ching Gong, Tung-Ming Hsiung. Phytoplankton pigment analysis by HPLC and its application inalgal community investigations[J]. Botanical Bulletia of Academy Sinica,2002,43:283-290.
[14] Furuya K, Hayashi M, Yabushita Y. HPLC Determination of Phytoplankton Pigments Using N,N-Dimethyl-formamide [J]. Journal of Oceanography,1998,54:199-203.
[15] Laurie V H, Crypstal S T. Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments[J]. Journal of Chromatography, 2001,910:31-49.
[16] Joanna S. Qualitative and quantitative analysis of Baltic phytoplankton pigments[J]. Oceanologia,2000,42 (4):449-471.
[17] Chen Jixin,Huang Bangqin,Jia Xiwei,et al. Community structure of marine ultraphytoplankton in xiamen water using photosynthetic pigments[J]. Marine Environmental Science,2003,22(3):16-21.[陈纪新,黄邦钦,贾锡伟,等.利用光合色素研究厦门海域超微型浮游植物群落结构[J].海洋环境科学,2003, 22(3):16-21.]
[18] Vinay P S, Constatin A R. Chloroplast biogenesis: Determination of the molar extinction coefficient of divinyl chlorophyll a and b and their pheophytin[J]. Analytic Biochemistry,1992,207:261-266.
[19] Gao Yahui, Yu Qiubo, Qi Yuzao, et al. Speries composition and ecological distribution of planktnic diatoms in the Changjiang river estuary during spring[J]. Chinese Journal of Applied Ecology,2003,14(7):1 044-1 048.[高亚辉,虞秋波,齐雨藻,等.长江口附近海域春季浮游硅藻的种类组成和生态分布[J].应用生态学报,2003,14(7)∶1 044-1 048.]
[20] Wu Yulin, Fu Yuena, Zhang Yongshan, et al. Phutoplankton distributionand its relation to the runoft in the Changjiang estuary[J]. Oceanologic Etlimaologia Sinia,2004,35(3):246-251.[吴玉霖,傅月娜,张永山,等.长江口海域浮游植物分布及其与径流的关系[J].海洋与湖沼,2004,35(3):246-251.]
[21] Wu Y L, Zhang Y S, Zhou C X. Phytoplankton distribution and community structure in the east China sea (ECS) continental shelf[J]. Chinese Journal of Oceanology and Limnology,2000,18(1):74-79.
[22] 林金美. 东海浮游甲藻类的分布[J].海洋学报,1994,(2):110-115.
[23] Lu Douding, Jeanette G. Study on taconomy and distribution of Ornithocercas in the East China Sea[J]. Donghai Marine Science,2001,19(3):11-18.[陆斗定, Jeanette G. 东海陆架黑潮区鸟尾藻的分类及其生态分布特点[J].东海海洋,2001,19(3):11-18.]
[24] Yang Qingliang. Characteristics of Trichodesmium distribution in waters over continental shelves of the South Huanghai Sea and the East China Sea[J]. Acta Oceanologica Sinica,1998,20(5):93-100.[杨清良.南黄海和东海陆架区束毛藻(Trichodesmium)的分布特征[J].海洋学报1998,20(5):93-100.]
[25] Veldhuis M J, Kraay G W. Phytoplankton in the subtrophic Atlantic Ocean: Towards a better assessment of biomass and composition[J]. Deep-Sea Research I, 2004, 51:507-530.
[26] Furuya K. Subsurface chlorophyll maximum in the tropical and subtropical western Pacific Ocean: Vertical profiles of phytoplankton biomass and its relationship with chlorophyll a and particulate organic carbon[J]. Marine Biology,1990,107:529-539.
[27] Chen Y L L. Summer phytoplankton community structure in the Kuroshio current-related upwelling northeast of Taiwan[J]. TAO,1992,3(3): 305-320 .
[28] Takahashi K, Fujitani N, Yanada M, et al. Five year long particle fluxes in the central subarctic Pacific and the Bering Sea[C]∥Tsunogai S,ed. Biogeochemical Processes in the North Pacific. Tokyo: Japan Marine Science Foundation, 1997:277-289.
[29] Yu Fei, Zang Jiaye, Guo Binghuo, et al. Some phenomena of the kuroshio intrasion in to Shelt area and shelf circulation of the East China Sea[J]. Advances in Marine Science,2002,20(3):22-28.[于非,臧家业,郭炳火,等.黑潮水入侵东海陆架及陆架环流的若干现象[J].海洋科学进展,2002,20(3): 22-28.]
[30] Huang L M, Jian W J, Song X Y, et al. Species diversity and distribution for phytoplankton of the Pearl River estuary during rainy and dry seasons[J]. Marine Pollution Bulletin,2004,49: 588-596.
[31] Zhu Genhai,Ning Xiuren, Cai Yuming, et al. Studies on species composition and abundance distribution of phytoplankton in the South China Sea[J]. Acta Oceanologica Sinica,2003,25:9-23.[朱根海,宁修仁,蔡昱明,等.南海浮游植物种类组成和丰度分布的研究[J].海洋学报,2003,25:9-23.]
[32] Cortes M Y, Bollmann J, Thierstein H R. Coccolithophore ecology at the HOT station ALOHA, Hawaii[J]. Deep-Sea Research II,2001,48:1 957-1 981.
[33] Millie D F,Paerl H W,Hurley J P. Microalgal pigment assessments using high-performance liquid chromatography: A synopsis of organismal and ecological applications[J]. Canadian Journal of Fisheries and Aquatic Sciences,1993,50:2 513-2 527.
[34] Millie D F,Kirkpatrick G J,Vinyard B T. Relating photosynthetic pigments and in vivo optical density spectra to irradiance for the Florida red-tide dinoflagellate Gymnodinium breve[J]. Marine Ecology Progress Series, 1995, 120:65-75.
[35] Jeffrey S W, Wright S W. Qualitative and quantitative HPLC analysis of SCOR reference algal cultures[C]∥Jeffrey S W, Mantoura R F C, Wright S W, eds. Phytoplankton Pigments in Oceanography. Paris:UNESCO Press, 1997:343-360.
[36] Goericke R, Montoya J P. Estimating the contribution of microalgal taxa to chlorophyll a in the field-variations of pigment ratios under nutrient- and light-limited growth[J]. Marine Ecology Progress Series,1998,169:97-112.
[37] Schluter L,Mohlenberg F,Havskum H,et al. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas:Testing the influence of light and nutrients on pigment/chlorophyll a ratios[J]. Marine Ecology Progress Series,2000,192:49-63.
[38] Mackey D J, Higgins H W, Mackey M D, et al. Algal class abundance in the western equatorial Pacific: Estimation from HPLC pigments using CHEMTAX[J]. Deep-Sea Research I,1998,45:1 441-1 468.
[39] Wright S W, Enden R L V D. Phytoplankton community structure and stocks in the East Antarctic marginal ice zone (BROKE survey, January March 1996) determined by CHEMTAX analysis of HPLC pigment signatures[J]. Deep-Sea Research II,2000,47:2 363-2 400.
/
| 〈 |
|
〉 |
甘公网安备62010202000687
