收稿日期: 2022-05-03
修回日期: 2022-09-14
网络出版日期: 2022-12-16
基金资助
国家自然科学基金项目“基于生物标志物和14C的东海沉积陆源有机质埋藏效率和机制研究”(41906032)
A Review of Techniques of Purification of Source-Specific Sterols for Compound-Specific Isotope Analysis and Applications
Received date: 2022-05-03
Revised date: 2022-09-14
Online published: 2022-12-16
Supported by
the National Natural Science Foundation of China “Burial efficiency and mechanistic controls of terrestrial organic matter in East China Sea: constrained by biomarker and 14C”(41906032)
甾醇类化合物作为一类来源明确的生物标志物，其碳氢同位素分析技术是古环境研究中很有价值的工具。甾醇的氢同位素值具有重建古水文环境和古海洋盐度的潜力，成为古气候研究中最佳的氢同位素分析目标物。利用气相色谱—稳定同位素比质谱仪获得高精度的同位素值需要目标分析物和邻近化合物之间有较高的分离度，即能排除邻近峰的影响。然而自然环境样品中的甾醇类化合物难以纯化，在一定程度上限制了甾醇单体同位素在古环境研究中的广泛应用。通过总结几种不断改进的制备液相色谱纯化制备甾醇类单体化合物的方法，综合介绍了甾醇类单体化合物纯化分析技术及其同位素应用的研究现状。在实验室成功分离甲藻甾醇和菜子甾醇的基础上，拟通过将我国边缘海沉积物中的甾醇单体化合物分离、纯化和富集，使其能够满足稳定氢同位素和碳同位素的测定要求，为我国边缘海环境参数重建研究提供技术支持。
李莉 , 于蒙 , 赵美训 . 甾醇类单体化合物纯化分析技术及其同位素应用的研究进展[J]. 地球科学进展, 2022 , 37(12) : 1223 -1231 . DOI: 10.11867/j.issn.1001-8166.2022.069
Source-specific biomarkers are the best targets for isotope analysis and paleoclimatic applications. Compound-specific hydrogen isotope (δ2H) measurements of sterols can be applied to the reconstruction of hydroclimatic conditions and paleo-sea surface salinity. Precise measurements using gas chromatography-isotope ratio mass spectrometry require a high baseline resolution between target analyte and adjacent compounds. However, isotope analysis of sterols for paleoclimatic applications is limited due to the difficulty in purifying complex natural samples. This study aimed to review several improved purification methods of source-specific sterols using liquid chromatography and the applications of compound-specific isotope analysis of sterols in lakes or seas. In addition, we successfully purified dinosterol and brassicasterol from the Chinese marginal sea surface sediments, meeting the requirements for δ2H and carbon isotope analysis. This study provides significant insights and technical support for the paleoenvironmental reconstruction in the Chinese marginal seas.
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