收稿日期: 2010-04-30
修回日期: 2010-06-21
网络出版日期: 2010-07-10
基金资助
中国科学院知识创新工程重要方向项目“西太平洋典型热液/冷泉系统与微生物生命过程”(编号：KZCX2-YW-211-03）；国家高技术研究发展计划（863计划）重点课题“大洋钻探站位调查关键技术研究”（编号：2008AA093001）资助.
Diversity of Achaea Communities in Deep Sea Sediments from the Sulu Sea
Received date: 2010-04-30
Revised date: 2010-06-21
Online published: 2010-07-10
为了研究热带西太平洋深海沉积物中古菌群落结构的多样性，应用16S rDNA文库技术，对IMAGES ⅪⅤ 航次采集的岩芯MD3 059进行基因文库的构建和分析，选取得到543个克隆，处理获得137个OTUs (Operational Taxonomic Units)。基于16S rRNA序列的同源性比较，绘制系统进化树，进行统计学分析。研究结果显示，热带西太平洋苏禄海沉积物中有丰富多样的古菌群落，在不足5 m的沉积物中垂直分布着11种古菌类群。古菌序列归属于泉古菌（Crenarchaeota）和广古菌(Euryarchaeota)，其中，前者主要由Miscellaneous Crenarchaeotic Group(MCG)构成（占总古菌克隆序列的50%以上），而后者主要由Marine Benthic Group D(MBG-D)、South Africa Gold Mine Euryarchaeotic Group(SAGMEG)和Marine Benthic Group B (MBG-B)等种属构成（占总古菌克隆序列的30%以上）。研究结果对于理解热带西太平洋深海沉积物中微生物多样性和未来开发利用微生物资源具有重要意义。
王剑飞，萨仁高娃，李铁刚，申之义，于心科 . 苏禄海深海沉积物古菌群落结构多样性研究[J]. 地球科学进展, 2010 , 25(7) : 766 -774 . DOI: 10.11867/j.issn.1001-8166.2010.07.0766
To study the archaeal diversity of deep-sea sediments in the tropical Western Pacific, we investigated the archaeal community structure in six sediments subsamples along the MD3059 sediment core collected during the IMAGES ⅪⅤ cruise with 16S rDNA analysis. A total of 543 archaeal 16S rDNA clones were examined and a total of 137 OTUs were obtained. Phylogenetic results showed that the archaeal diversity in the collected samples was very diverse, and the obtained OTUs were grouped into Crenarchaeota and Euryarchaeota, with the former dominated by Miscellaneous Crenarchaeotic Group (MCG, 54% of total archaeal clones) and the latter dominated by Marine Benthic Group D (MBG-D), South African Gold Mine Euryarchaeotic Group (SAGMEG) and Marine Benthic Group B (MBG-B). These data have important implications for our understanding of archaeal community in deep-sea sediments of the tropical Western Pacific.
Key words： The Sulu Sea; Marine sediment; Achaea; Distribution
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