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地球科学进展  2010, Vol. 25 Issue (4): 360-364    DOI: 10.11867/j.issn.1001-8166.2010.04.0360
张翠云,张俊霞,马琳娜,张  胜,殷密英,李政红
中国地质科学院水文地质环境地质研究所,河北  石家庄  050061
Advances in the Study of the Denitrifier Method for the Measurement of Isotopic Compositions of Nitrate
Zhang Cuiyun,Zhang Junxia,Ma Linna,Zhang Sheng,Yin Miying,Li Zhenghong
Institute of Hydrogeology & Environmental Geology, Chinese Academy of Geology Sciences, Shijiazhuang  050061, China
 全文: PDF(1050 KB)  


关键词: 氮氧同位素反硝化细菌法测试技术    

 In the 21st century, the significant advances in the measurement of nitrogen and oxygen isotopic compositions of nitrate are the development of analytical techniques of the denitrifier method. The method has many advantages, such as simultaneous analysis of nitrogen and oxygen isotopic compositions of samples with low NO3- concentrations down to μg/L, relief from complicated sample pretreatment, a large reduction in analysis time, only conversion of NO3- in samples to N2O in complex solutions and so on. The experimental procedure consists of several key steps including bacterium selection and cultivation, conversion of sample NO3- to N2O, extraction and isotopic analysis of N2O and correction of isotopic analytical results. Results from foreign researchers showed that the technique was applicable for the measurement from soil, seawater and freshwater, and was widely used. This technique was also developed in and obtained important advances in the contamination study of nitrate in the vadose zone and groundwater. Because of its advanced analytical technique, We suggest great efforts be given to its research and application in China. Concerned agencies in charge should give more support to the study so that the technique can be modified further and applied widely.

Key words: The denitrifier method    Nitrogen and oxygen isotopes    Analytical techniques
收稿日期: 2009-09-01 出版日期: 2010-04-10
:  P595  

国家自然科学基金项目“反硝化细菌法测定水土环境中硝酸盐氮氧同位素的研究”(编号:40472154);地质调查项目下属专题“污灌区水土污染自然衰减调查评价”(编号:200310400034)资助.  

通讯作者: 张翠云     E-mail:
作者简介: 张翠云(1962-),女, 广东兴宁人,博士,研究员,主要从事地质微生物和同位素研究.
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张翠云,张俊霞,马琳娜,张胜,殷密英,李政红. 硝酸盐氮氧同位素反硝化细菌法测试研究[J]. 地球科学进展, 2010, 25(4): 360-364.

Zhang Cuiyun,Zhang Junxia,Ma Linna,Zhang Sheng,Yin Miying,Li Zhenghong. Advances in the Study of the Denitrifier Method for the Measurement of Isotopic Compositions of Nitrate. Advances in Earth Science, 2010, 25(4): 360-364.


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