地球科学进展 ›› 2010, Vol. 25 ›› Issue (4): 360 -364. doi: 10.11867/j.issn.1001-8166.2010.04.0360

综述与评述 上一篇    下一篇

硝酸盐氮氧同位素反硝化细菌法测试研究
张翠云,张俊霞,马琳娜,张  胜,殷密英,李政红   
  1. 中国地质科学院水文地质环境地质研究所,河北  石家庄  050061
  • 收稿日期:2009-09-01 修回日期:2010-01-25 出版日期:2010-04-10
  • 通讯作者: 张翠云 E-mail:cuiyunzhang2000@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目“反硝化细菌法测定水土环境中硝酸盐氮氧同位素的研究”(编号:40472154);地质调查项目下属专题“污灌区水土污染自然衰减调查评价”(编号:200310400034)资助.  

Advances in the Study of the Denitrifier Method for the Measurement of Isotopic Compositions of Nitrate

Zhang Cuiyun,Zhang Junxia,Ma Linna,Zhang Sheng,Yin Miying,Li Zhenghong   

  1. Institute of Hydrogeology & Environmental Geology, Chinese Academy of Geology Sciences, Shijiazhuang  050061, China
  • Received:2009-09-01 Revised:2010-01-25 Online:2010-04-10 Published:2010-04-10
  • Contact: Zhang CuiYun E-mail:cuiyunzhang2000@yahoo.com.cn
  • Supported by:

    The denitrifier method for determination of nitrogen and oxygen isotopes of nitrate in groundwater and soil

 进入21世纪,硝酸盐氮氧同位素测试技术的显著进展是反硝化细菌法测试技术的建立。反硝化细菌法具有可同时分析浓度低至μg/L的硝酸盐氮氧同位素组成、免去复杂的样品预处理、分析时间大大缩短以及从复杂溶液中只转化NO3-为N2O等优点。实验过程由细菌挑选和培养、样品NO3-转化为N2O、N2O提取纯化、同位素测试以及同位素测试结果校正等几个关键步骤组成。国外研究结果显示该技术不仅可用于海水和淡水中硝酸盐同位素测试,而且还可用于土壤样品,并得到不断的完善和应用|国内建立了这一技术,在包气带和地下水硝酸盐污染研究中取得重要进展。由于该技术诸多优点,建议大力促进这一技术在我国的研究和应用,希望有关部门加大支持力度,进一步完善该技术并推广应用。

 In the 21st century, the significant advances in the measurement of nitrogen and oxygen isotopic compositions of nitrate are the development of analytical techniques of the denitrifier method. The method has many advantages, such as simultaneous analysis of nitrogen and oxygen isotopic compositions of samples with low NO3- concentrations down to μg/L, relief from complicated sample pretreatment, a large reduction in analysis time, only conversion of NO3- in samples to N2O in complex solutions and so on. The experimental procedure consists of several key steps including bacterium selection and cultivation, conversion of sample NO3- to N2O, extraction and isotopic analysis of N2O and correction of isotopic analytical results. Results from foreign researchers showed that the technique was applicable for the measurement from soil, seawater and freshwater, and was widely used. This technique was also developed in and obtained important advances in the contamination study of nitrate in the vadose zone and groundwater. Because of its advanced analytical technique, We suggest great efforts be given to its research and application in China. Concerned agencies in charge should give more support to the study so that the technique can be modified further and applied widely.

中图分类号: 

[1] Showers W J, Genna B, McDade T, et al. Nitrate contamination in groundwater on an urbanized dairy farm[J]. Environmental Science & Technology,2008, 42:4 683-4 688.
[2] Huang Tinglin, Chai Beibei. Advances in the study of controlling mechanics and technology for source water reservoir quality[J]. Advances in Earth Science,2009, 24(6):588-596.[黄廷林,柴蓓蓓.水源水库水质污染 与富营养化控制技术研究进展[J].地球科学进展, 2009,24(6):588-596.]
[3] Bremner J M, Edwards A P. Determination and isotope-ratio analysis of different forms of nitrogen in soils: I. Apparatus and procedure for distillation and determination of ammonium[J].Soil Science Society of America Proceedings,1965, 29:504-507.
[4] MacKown C T, Brooks P D, Smith M S. Diffusion of Nitrogen-15 Kjeldahl digests for isotope analysis[J].Soil Science Society of America Journal,1987, 51: 87-90.
[5] Sigman D M, Altabet M A, Michener R, et al. Natural abundance-level measurement of the nitrogen isotopic composition of oceanic nitrate: An adaptation of the ammonia diffusion method[J].Marine Chemistry,1997, 57:227-242.
[6] Cao Yacheng, Sun Guoqing, Shi Shulian. Determination of δ15N of different forms of nitrogen in soils by mass spectrometry[J].Chinese Journal of Soil Science,1993,24(2):87-90.[曹亚澄,孙国庆,施书莲.土壤中不同含氮组份的15N质谱测定法[J].土壤通讯,1993,24(2):87-90.]
[7] Kendall C, Grim E. Combustion tube method for measurement of nitrogen isotope ratios using calcium oxide for total removal of carbon dioxide and water[J].Analytical Chemistry,1990, 62: 526-529.[8] Revesz K, Bhlk J K, Yoshinari T. Determination of 18O and 15N in nitrate[J].Analytical Chemistry,1997, 69: 4 375-4 380.
[9] Chang C C Y, Langston J, Riggs M, et al. A method for nitrate collection for delta 15N and delta 18O analysis from waters with low nitrate concentrations[J].Canadian Journal of Fisheries and Aquatic Sciences,1999,56:1 856-1 864.
[10] Silva S R, Kendall C, Wilkinson D H, et al. A new method for collection of nitrate from fresh water and the analysis of nitrogen and oxygen isotope ratios[J].Journal of Hydrology,2000,228:22-36.
[11] Yang Yan , Cai Hesheng, Liu Cunfu, et al. A new analytical technique for detecting isotopes 15N and 18O in NO3- of groundwater: Sealed combustion quartz tube method[J].Hydrogeology and Engineering Geology,2005, (2): 20-24.[杨琰, 蔡鹤生, 刘存富,等.地下水中NO3-15N和18O同位素测试新技术—密封石英管燃烧法[J]. 水文地质工程地质,2005, (2): 20-24.]
[12] Christensen S, Tiedje J M. Sub-parts-per-billion nitrate method: Use of an N2O-producing denitrifier to convert NO3- or 15NO3- to N2O[J].Applied and Environmental Microbiology,1988,54(6): 1 409- 1 413.
[13] Sigman D M,Casciotti K L,Andreani M, et al. A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater[J].Analytical Chemistry,2001, 73: 4 145-4 153.
[14] Casciotti K L, Sigman D M, Hastings M G,et al. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method[J].Analytical Chemistry,2002, 74:  4 905-4 912.
[15] Mørkved P T, Dörsch P, Søvik A K,et al. Simplified preparation for the δ15N-analysis in soil NO3- by the denitrifier method[J].Soil Biology and Biochemistry,2007,39: 1 907-1 915.
[16] Rock L, Ellert B. Nitrogen-15 and Oxygen-18 natural abundance of potassium chloride extractable soil nitrate using the denitrifier method, A new method for collection of nitrate from fresh water and the analysis of Nitrogen and Oxygen Isotope Ratios[J].Soil Science Society of America Journal, 2007,71(2): 355-361.
[17] Casciotti K L, Böhlke J K, McIlvin M R, et al. Oxygen isotopes in nitrite: Analysis, calibration, and equilibration[J].Analytical Chemistry, 2007,79:2 427-2 436.
[18] Casciotti K L, McIlvin M R. Isotopic analyses of nitrate and nitrite from reference mixtures and application to Eastern Tropical North Pacific waters[J].Marine Chemistry,2007,107(2): 184-201.
[19] Granger J,Sigman D M,Prokopenko M G, et al. A method for nitrite removal in nitrate N and O isotope analyses[J].Limnology and Oceanography-Methods,2006, 4: 205-212.
[20] Coplen T B, Bohlke J K, Casciotti K L. Using dual bacterial denitrification to improve 15N determinations of nitrates containing mass independent 17O[J]. Rapid Communications in Mass Spectrometry, 2004,18: 245-250.
[21] Ohte N, Sebestyen S D, Shanley J B, et al. Tracing sources of nitrate in snowmelt runoff using a high-resolution isotopic technique[J]. Geophysical Research Letters, 2004,31(21): 4.
[22] Lourey M J, Trull T W, Sigman D M. Sensitivity of 15N of nitrate, surface suspended and deep sinking particulate nitrogen to seasonal nitrate depletion in the Southern Ocean[J]. Global Biogeochemical Cycles, 2003,17(3): 1 081.
[23] Lehmann M F, Sigman D M, McCorkle D C, et al. The distribution of nitrate 15N/14N in marine sediments and the impact of benthic nitrogen loss on the isotopic composition of oceanic nitrate[J].Geochimica et Cosmochimica Acta,2007, 71: 5 384-5 404.
[24] Sigman D M, Robinson R, Knapp A N, et al. Distinguishing between water column and sedimentary denitrification in the Santa Barbara Basin using the stable isotopes of nitrate[J].Geochemistry, Geophysics, Geosystems,2003, 4: 1-20.

[1] 李朝阳. 对当前矿床地球化学研究的一点认识[J]. 地球科学进展, 1999, 14(6): 549-554.
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