Western Liao River area is one of the most important birthplaces of agricultural civilization in northern China. With recent works like relics survey, site excavation and environmental archaeology going further, the time sequence, cultural content and subsistence strategy history of this area are getting explicit. Questions like ancient exploiting history of natural resources, climate background and cultural impact of subsistence strategy have been paid great attention. Traditional environmental archaeology methods like plant and animal remains analysis are mostly utilized to conduct in-site investigation, current studies rarely adopt quantitative methods to examine the sites’ macro temporal-spatial distribution and aggregation pattern. Based on abundant digitized relic survey data, GIS methods like kernel density analysis are utilized to indicate the temporal-spatial distribution and aggregation pattern of prehistoric cultures in Western Liao River area. The latitudinal, longitudinal and altitudinal migrations of site distributional core are indicated by 3D perspective shift and elevation statistics. By referencing existing studies on subsistence strategy and paleoclimate history, results of spatial analysis are used to reveal the correlation among sites’ temporal-spatial distribution, subsistence strategy adopted and climate variation. This also contributes to our understanding and utilization of massive locational information of prehistoric sites in ancient man-land relationship study.