Since industrial revolution, greenhouse gases concentrations at atmosphere increased continually. Some new progresses in the study on concentration change of major greenhouse gases have been achieved from ice-core record such as last four glacial-interglacial cycles record of carbon dioxide concentration. New knowledge concerning to early Holocene carbon dioxide concentration, concentration variation of methane during Holocene and abrupt change of nitrous oxide concentration have been revealed. These new results present good choice to study relationship between concentration variation of greenhouse gases and climate change, which can help to understand mechanism of climate change. To study source and sink of greenhouse gases is basis for prediction of future climate change. The missing sink of global carbon cycle attracted more attention. According to different models and data, there is large difference on magnitude and location of estimated carbon sink in terrestrial biosphere. In China, some studies on greenhouse gases emission and sequestration such as methane emission from rice paddy, carbon cycle in karst processes and feature of major greenhouse gases in loess have been carried out.